Influence of virus and virus-like agents on the development of citrus buds cultured in vitro
- Cite this article as:
- Greño, V., Navarro, L. & Duran-Vila, N. Plant Cell Tiss Organ Cult (1988) 15: 113. doi:10.1007/BF00035753
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Tissue culture in vitro was used to determine the effect of six major citrus virus and virus-like agents. Nodal stem segments from inoculated Pineapple sweet orange (Citrus sinensis (L.) Osb.), Mexican lime (C. aurantifolia (Christm.) Swing.) and Arizona Etrog citron 861-Sl (C. medica L.) were cultured in vitro to induce shoots. Some virus and virus-like agents had a marked effect on bud development and further recovery of plantlets. The number and size of the shoots that developed from each bud were affected as a result of infection. The effect depended on the specific virus, the isolate and the host-disease combination. The possible implications of these results are discussed.