Plant Cell, Tissue and Organ Culture

, Volume 25, Issue 1, pp 75–84

The role of abscisic acid and plant growth regulators in tissue culture-induced rejuvenation of strawberry ex vitro

  • Fouad Mohamed
  • Harry Jan Swartz
  • J. George Buta
Article

DOI: 10.1007/BF00033916

Cite this article as:
Mohamed, F., Swartz, H.J. & Buta, J.G. Plant Cell Tiss Organ Cult (1991) 25: 75. doi:10.1007/BF00033916

Abstract

Plant growth regulators applied in vitro affected strawberry plant performance ex vitro for a period of up to 4 months. Benzyl-adenine and gibberellin enhanced juvenile characteristics; in general, more runners and monofoliolate leaves were produced, net photosynthesis was reduced, leaf diffusive resistance was increased and, on occasion, flowering was delayed. In contrast, abscisic acid and a gibberellin biosynthesis inhibitor, paclobutrazol, resulted in a more adult phenotype; specifically, flowering was earlier, net photosynthesis and leaf diffusive resistance rates were equal to those of adult plants, and fewer leaves were monofoliolate. Tissue culture-produced plants reacquire the adult phenotype earlier than seedlings mature. Abscisic acid application to seedlings also resulted in earlier and increased flowering.

Endogenous abscisic acid concentrations were greater in adult plants and less in tissue culture and seedling plants at 3 and 7 weeks ex vitro, after germination or after adult runner propagation. No flowering occurred at 3 weeks in any propagation type and only runner-produced plants flowered at 7 weeks. At 15 weeks, no statistical difference in abscisic acid concentrations existed among propagation treatments and all propagation types flowered. The endogenous concentrations of abscisic acid in plants propagated by all three methods were much higher at three weeks compared to concentrations at 7 or 15 weeks.

Key words

benzyladeninefloweringFragariagibberellingrowth retardantjuvenllity

Abbreviations

ABA

+cis, trans-abscisic acid

BA

benzyl-adenine

GC-SIM-MS

combined gas chromatography-selected ion monitoring mass spectrometry

GA

gibberellin A3

RS

leaf diffusive resistance in sec cm-1

MS

Murashige & Skoog medium devoid of plant growth regulators

Pn

net photosynthesis in μmol CO2 fixed m-2 sec-1

paclo

paclobutrazol as 50WP

TC-plants

plants produced from tissue culture

Tween20

polyoxyethylene-sorbitan monolaurate

RP

runner plants

SDLG

selfed seedlings

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Fouad Mohamed
    • 1
  • Harry Jan Swartz
    • 1
  • J. George Buta
    • 2
  1. 1.Department of HorticultureUniversity of MarylandCollege ParkUSA
  2. 2.U.S. Department of AgriculturePlant Hormone LaboratoryBeltsvilleUSA