Plant Molecular Biology

, Volume 24, Issue 5, pp 743–755

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)

Authors

  • Francesca Sparvoli
    • Dipartmento di Genetica e di Biologia dei MicroorganismiUniversità degli Studi di Milano
  • Cathie Martin
    • Department of GeneticsJohn Innes Institute
  • Attilio Scienza
    • Istituto di Coltivazioni ArboreeUniversità degli Studi di Milano
  • Giuseppe Gavazzi
    • Dipartmento di Genetica e di Biologia dei MicroorganismiUniversità degli Studi di Milano
  • Chiara Tonelli
    • Dipartmento di Genetica e di Biologia dei MicroorganismiUniversità degli Studi di Milano
Research Articles

DOI: 10.1007/BF00029856

Cite this article as:
Sparvoli, F., Martin, C., Scienza, A. et al. Plant Mol Biol (1994) 24: 743. doi:10.1007/BF00029856

Abstract

Genes involved in flavonoid and stilbene biosynthesis were isolated from grape (Vitis vinifera L.). Clones coding for phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydoxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) and UDP glucose:flavonoid 3-O-glucosyl transferase (UFGT), were isolated by screening a cDNA library, obtained from mRNA from seedlings grown in light for 48 h using snapdragon (Antirrhinum majus) and maize heterologous probes. A cDNA clone coding for stilbene synthase (StSy) was isolated by probing the library with a specific oligonucleotide. These clones were sequenced and when the putative products were compared to the published amino acid sequence for corresponding enzymes, the percentages of similarity ranged from 65% (UFGT) to 90% (CHS and PAL). The analysis of the genomic organization and expression of these genes in response to light shows that PAL and StSy genes belong to large multigene families, while the others are present in one to four copies per haploid genome. The steady-state level of mRNAs encoded by the flavonoid biosynthetic genes as determined in young seedlings is coordinately induced by light, except for PAL and StSy, which appear to be constitutively expressed.

Key words

anthocyaninscDNA cloningflavonoidsgene expressiongenomic organizationstilbenesVitis vinifera L

Copyright information

© Kluwer Academic Publishers 1994