Plant Molecular Biology

, Volume 25, Issue 4, pp 739–742

Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding

  • N. Harris
  • J. E. Taylor
  • J. A. Roberts
Short Communication

DOI: 10.1007/BF00029611

Cite this article as:
Harris, N., Taylor, J.E. & Roberts, J.A. Plant Mol Biol (1994) 25: 739. doi:10.1007/BF00029611

Abstract

A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.

Key words

Lycopersicon esculentumtomatonucleoside diphosphate kinasewoundingethylene

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • N. Harris
    • 1
  • J. E. Taylor
    • 1
  • J. A. Roberts
    • 1
  1. 1.Department of Physiology and Environmental Science, Faculty of Agricultural and Food SciencesUniversity of NottinghamLoughboroughUK
  2. 2.Division of Biological Sciences, Institute of Enviromental and Biological SciencesLancaster UniversityLancasterUK