Plant Molecular Biology

, Volume 25, Issue 4, pp 705–719

A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare

  • Nami Okumura
  • Naoko-Kishi Nishizawa
  • Yosuke Umehara
  • Tomoko Ohata
  • Hiromi Nakanishi
  • Takahiro Yamaguchi
  • Mitsuo Chino
  • Satoshi Mori
Research Article

DOI: 10.1007/BF00029608

Cite this article as:
Okumura, N., Nishizawa, NK., Umehara, Y. et al. Plant Mol Biol (1994) 25: 705. doi:10.1007/BF00029608

Abstract

A λzapII cDNA library was constructed from mRNA isolated from Fe-deficient barley roots and screened with cDNA probes made from mRNA of Fe-deficient and Fe-sufficient (control) barley roots. Seven clones were selected. Among them a clone having the putative full-length mRNA of dioxygenase as judged by northern hybridization was selected and named Ids2 (iron deficiency-specific clone 2). Using a cDNA fragment as probe, two clones from the genomic library (λEMBL-III) were isolated and one was sequenced. The predicted amino acid sequence of Ids2 resembled that of 2-oxoglutarate-dependent dioxygenase. Ids2 is expressed in the Fe-deficient barley roots but is not in the leaves. The expression is repressed by the availability of Fe. Ids2 was also strongly expressed under Mn deficiency and weakly under Zn deficiency or excess NaCl (0.5%). The upstream 5′-flanking region of Ids2 has a root-specific cis element of the CaMV 35S promoter and a nodule-specific element of leghemoglobin, a metal regulatory element (MRE) and several Cu regulatory elements (UAS) of yeast metallothionein (CUP1).

Key words

chlorosisDNA sequenceIds2iron deficiencyMAsphytosiderophores

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Nami Okumura
    • 1
  • Naoko-Kishi Nishizawa
    • 1
  • Yosuke Umehara
    • 1
  • Tomoko Ohata
    • 1
  • Hiromi Nakanishi
    • 1
  • Takahiro Yamaguchi
    • 1
  • Mitsuo Chino
    • 1
  • Satoshi Mori
    • 1
  1. 1.Laboratory of Plant Nutrition and FertilizerUniversity of TokyoTokyoJapan