Plant Molecular Biology

, Volume 26, Issue 3, pp 781–790

Reduced ethylene synthesis by transgenic tomatoes expressing S-adenosylmethionine hydrolase

  • Xin Good
  • Jill A. Kellogg
  • Wendy Wagoner
  • Dan Langhoff
  • Wendy Matsumura
  • Richard K. Bestwick
Research Articles

DOI: 10.1007/BF00028848

Cite this article as:
Good, X., Kellogg, J.A., Wagoner, W. et al. Plant Mol Biol (1994) 26: 781. doi:10.1007/BF00028848

Abstract

We have utilized a gene from bacteriophage T3 that encodes the enzyme S-adenosylmethionine hydrolase (SAMase) to generate transgenic tomato plants that produce fruit with a reduced capacity to synthesize ethylene. S-adenosylmethionine (SAM) is the metabolic precursor of 1-aminocyclopropane-1-carboxylic acid, the proximal precursor to ethylene. SAMase catalyzes the conversion of SAM to methylthioadenosine and homoserine. To restrict the presence of SAMase to ripening fruit, the promoter from the tomato E8 gene was used to regulate SAMase gene expression. Transgenic tomato plants containing the 1.1 kb E8 promoter bore fruit that expressed SAMase during the breaker and orange stage of fruit ripening and stopped expression after the fruit fully ripened. Plants containing the 2.3 kb E8 promoter expressed SAMase at higher levels during the post-breaker phases of fruit ripening and had a substantially reduced capacity to synthesize ethylene.

Key words

EthyleneripeningS-adenosylmethionine hydrolaseSAMasetomatotransgenic

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Xin Good
    • 1
  • Jill A. Kellogg
    • 1
  • Wendy Wagoner
    • 1
  • Dan Langhoff
    • 1
  • Wendy Matsumura
    • 1
  • Richard K. Bestwick
    • 1
  1. 1.Agritope, Inc.BeavertonUSA