Plant Molecular Biology

, Volume 11, Issue 3, pp 277–291

Sequence and characterization of 6 Lea proteins and their genes from cotton

  • Jean Baker
  • Christopher Van dennSteele
  • Leon DureIII
Article

DOI: 10.1007/BF00027385

Cite this article as:
Baker, J., Van dennSteele, C. & Dure, L. Plant Mol Biol (1988) 11: 277. doi:10.1007/BF00027385

Abstract

Lea genes code for mRNAs and proteins that are late embryogenesis abundant in higher plant seed embryos. They appear to be ubiquitous in higher plants and may be induced to high levels of expression in other tissues and at other times of ontogeny by ABA and/or desiccation. Presented here are the genomic and cDNA sequences for 6 of these genes from cotton seed embryos and the derived amino acid sequences of the corresponding proteins.

The Lea genes contain the standard sequence features of eucaryotic genes (TATA box and poly (A) addition sequences) and have 1 or more introns. Sequences differences between cDNA and genomic DNA confirm the existence of small multigene families for several Lea genes. The amino acid composition and sequence for the Lea proteins are unusual. Five are extremely hydrophilic, four contain no cys or trp and 4 have sequence domains that suggest amphiphilic helical structures. Hypothetical functions in desiccation survival, based on amino acid sequence, are discussed.

Key words

cotton proteinscotton genesLea proteinsLea genesabscisic acidplant desiccation

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Jean Baker
    • 1
  • Christopher Van dennSteele
    • 1
  • Leon DureIII
    • 1
  1. 1.Department of BiochemistryUniversity of GeorgiaAthensUSA