Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents
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We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79 000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equivalents. The library is organised as an ordered array in duplicate microtitre plates. Forty-one pools of DNA from 1920 individual clones have been prepared for rapid screening of the library by the polymerase chain reaction (PCR). Using this approach, together with conventional colony hybridisation, we have been able to identify between one and eight positive clones for every probe used.
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- Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents
Plant Molecular Biology
Volume 19, Issue 2 , pp 299-308
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- cloning in YACs
- genome mapping
- Industry Sectors
- Author Affiliations
- 1. Plant Biotechnology Section, I.C.I. Seeds, Jealotts Hill Research Station, RG12 6EY, Bracknell, Berkshire, UK