Plant Molecular Biology

, Volume 19, Issue 4, pp 699–703

A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II

Authors

  • Jakob Brandt
    • Section for Plant Pathology, Department of Plant BiologyThe Royal Veterinary and Agricultural University
  • Vibeke Skovgaard Nielsen
    • Plant Biochemistry Laboratory, Department of Plant BiologyThe Royal Veterinary and Agricultural University
  • Hans Thordal-Christensen
    • Section for Plant Pathology, Department of Plant BiologyThe Royal Veterinary and Agricultural University
  • David John Simpson
    • Department of PhysiologyCarlsberg Laboratory
  • Jens Sigurd Okkers
    • Plant Biochemistry Laboratory, Department of Plant BiologyThe Royal Veterinary and Agricultural University
Update Section Short Communication

DOI: 10.1007/BF00026796

Cite this article as:
Brandt, J., Nielsen, V.S., Thordal-Christensen, H. et al. Plant Mol Biol (1992) 19: 699. doi:10.1007/BF00026796

Abstract

The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LHCII) in barley is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.

Key words

Type III LHCIICAB proteinnucleotide sequenceprotein importlight inductionHordeum vulgare

Copyright information

© Kluwer Academic Publishers 1992