Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons
- Cite this article as:
- Jayabaskaran, C. Plant Growth Regul (1990) 9: 9. doi:10.1007/BF00025274
A protein which binds specifically to [3H]-zeatin has been isolated from cucumber cotyledons by chromatographic techniques. Its binding to [3H]-zeatin was inhibited remarkably by the addition of non-radioactive cytokinins and the order of inhibition was zeatin > -zeatin riboside > N6-(Δ2-isopentenyl)adenine > N6-(Δ2-isopentenyl)adenosine > N6-benzyl-adenosine > kinetin riboside. This protein behaved as a soluble protein with an apparent molecular size of 43,000 daltons on gel filtration through calibrated Sephadex G-100 column. The dissociation constant, Kd, of the protein-zeatin complex was about 4 × 10−7 M.