Repeat sequence interspersion in coding DNA of peas does not reflect that in total pea DNA
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The pattern of sequence organization in the regions of the pea genome near sequences coding for mRNA differs significantly from that in total DNA. Interspersion of repeated and single copy sequences is so extensive that 85% of 1300 nucleotide-long fragments contain highly repetitive sequences (about 5000 copies per haploid genome). However, data presented here demonstrate that sequences which code for mRNA are enriched in the small fraction of fragments which do not contain these highly repetitive sequences. Thus, in contrast to the great majority of other sequences in the genome, most mRNA coding sequences are not located within 1300 nucleotides of highly repetitive elements. Moreover, our data indicate that those repeats (if any) which are closely associated with mRNA coding sequences belong to low copy number families characterized by an unusually low degree of sequence divergence.
- Adams JW, Kaufman RE, Kretschmer PJ, Harrison M and Nienhuis AW (1980) A family of long reiterated DNA-sequences, one copy of which is next to the human beta globin gene. Nuc Acids Res 8, 6113–6128.
- Angerer RC, Davidson EH and Britten RJ (1976) Single copy DNA and structural gene sequences relationship among four sea urchin species. Chromosoma 56, 213–226.
- Bennett MD and Smith JB (1976) Nuclear DNA amounts in Angiosperms. Philos Trans R Soc London Ser. B 274, 227–273.
- Britten RJ, and Davidson EH (1969) Gene regulation for higher cells: a theory. Science 165, 349–357.
- Britten RJ, Cetta A and Davidson EH (1978) The single copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus. Cell 15, 1175–1186.
- Britten RJ, Graham DE and Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. Methods Enzymol 29, 364–418.
- Coggins LW, Grindlay GJ, Vass JK, Slater SS, Montague P, Stinson MA, and Paul J (1980) Repetitive sequences near three human β-type globin genes. Nuc Acids Res 8, 3319–3333.
- Costantini FD, Britten RJ and Davidson EH (1980) Message sequences and short repetitive sequences are interspersed in sea urchin egg poly (A) + RNAs. Nature 287, 111–117.
- Cuellar RE, Ford GA, Briggs WR and Thompson WF (1978) Application of higher derivative techniques to analysis of high-resolution thermal denaturation profiles of reassociated repetitive DNA. Proc Natl Acad Sci 75, 6026–6030.
- Davidson EH and Britten RJ (1979) Regulation of gene expression: possible role of repetitive sequences. Science 204, 1052–1059.
- Davidson EH, Hough BR, Klein WH, Britten RJ (1975) Structural genes adjacent to interspersed repetitive sequences. Cell 4, 217–238.
- Dumas LB, Darby G, and Sinsheimer RL (1971) The replication of bacteriophage øX 174 in vitro temperature effects of repair synthesis and displacement synthesis. Biochim Biophys Acta 229, 407–422.
- Duncan C, Biro RA, Choudary PV, Elder JT, Wang RRC, Forget EG, De Riel JK and Weissman SA (1979) RNA polymerase III transcription units are interspersed among human non-α-globin genes. Proc Natl Acad Sci 76, 5095–5099.
- Kuroiwa A and Natori S (1979) Preferential expression of unique sequences adjacent to middle repetitive sequences in mouse cytoplasmic RNA. Nuc Acids Res 7, 751–764.
- McDonell MW, Simon MN and Studier FW (1977) Analysis of restriction fragments of T-7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 110, 119–146.
- Murray MG, Cuellar RE and Thompson WF (1978) DNA sequence organization in the pea genome. Biochemistry 17, 5781–5790.
- Murray MG, Peters DL and Thompson WF (1981) Ancient repeated sequences in the pea and mung bean genomes and implications for genome evolution. J Mol Evol 17, 31–42.
- Murray MG, Palmer JD, Cuellar RE and Thompson WF (1979) DNA sequence organization in the mung bean genome. Biochemistry 18, 5259–5266.
- Pearson WR, Davidson EH and Britten RJ (1977) A program for least squares analysis of reassociation and hybridization data. Nuc Acids Res 4, 1727–1735.
- Preisler RS and Thompson WF (1981) Evolutionary sequence divergence within repeated DNA families of higher plant genomes. II. Analysis of thermal denaturation. J. Mol Evol 17: 85–93.
- Thompson WF (1976) Aggregate formation from short fragments of plant DNA. Plant Physiol 57, 617–622.
- Thompson WF and Murray MG (1981) The nuclear genome: structure and function. In Marcus A, ed. The Biochemistry of Plants Vol 6, pp 1–81, New York, N.Y.: Academic Press.
- Wetmur JG (1976) Hybridization and renaturation kinetics of mucleic acids. Ann Rev Biophys Bioeng 5, 337–361.
- Repeat sequence interspersion in coding DNA of peas does not reflect that in total pea DNA
Plant Molecular Biology
Volume 1, Issue 2 , pp 143-153
- Cover Date
- Print ISSN
- Online ISSN
- Martinus Nijhoff/Dr. W. Junk Publishers
- Additional Links
- Repeated DNA
- sequence divergence
- Industry Sectors