Euphytica

, Volume 85, Issue 1, pp 173–180

Synergistic activity of chitinases and β-1,3-glucanases enhances fungal resistance in transgenic tomato plants

  • Erik Jongedijk
  • Henk Tigelaar
  • Jeroen S. C. van Roekel
  • Sandra A. Bres-Vloemans
  • Ilma Dekker
  • Peter J. M. van den Elzen
  • Ben J. C. Cornelissen
  • Leo S. Melchers
Article

DOI: 10.1007/BF00023946

Cite this article as:
Jongedijk, E., Tigelaar, H., van Roekel, J.S.C. et al. Euphytica (1995) 85: 173. doi:10.1007/BF00023946

Summary

Simultaneous expression of a tobacco class I chitinase and a class I β-1,3-glucanase gene in tomato resulted in increased fungal resistance, whereas transgenic tomato plants expressing either one of these genes were not protected against fungal infection. After infection with Fusarium oxysporum f.sp. lycopersici, a 36% to 58% reduction in disease severity was observed in resistant tomato lines. Two transgenic lines largely recovered from the initial infection by the time wild-type tomato plants had died.

The overall results are consistent with the observation that class I chitinases and class I β-1,3-glucanases synergistically inhibit the growth of fungi in vitro and provide the first experimental support to the hypothesis that such synergy can contribute to enhanced fungal resistance in planta.

Key words

Lycopersicon esculentumtomatoendochitinasesβ-1,3-endoglucanasesfungal resistancetransgenic plants

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Erik Jongedijk
    • 1
  • Henk Tigelaar
    • 1
  • Jeroen S. C. van Roekel
    • 1
  • Sandra A. Bres-Vloemans
    • 1
  • Ilma Dekker
    • 1
  • Peter J. M. van den Elzen
    • 1
  • Ben J. C. Cornelissen
    • 2
  • Leo S. Melchers
    • 1
  1. 1.MOGEN International nvLeidenThe Netherlands
  2. 2.Institute for Molecular Cell BiologyBiocentrum AmsterdamAmsterdamThe Netherlands