Skip to main content
Log in

Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chiriboga J., 1966. Purification and properties of oxalic acid oxidase. Arch. Biochem. Biophys. 116: 516–523.

    Article  PubMed  CAS  Google Scholar 

  • Dumas B., A. Sailland, J-P. Cheviet, G. Freyssinet & K. Pallet, 1993. Identification of barley oxalate oxidase as a germin-like protein. C.R. Acad. Sci. Paris 316: 793–798.

    PubMed  CAS  Google Scholar 

  • Godoy G., J.R. Steadman, M.B. Dickman & R. Dam, 1990. Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiol. Mol. Plant Pathol. 37: 179–191.

    Article  CAS  Google Scholar 

  • Lane B.G., 1994. Oxalate, germin and the extracellular matrix. FASEB J. 8: 294–301.

    PubMed  CAS  Google Scholar 

  • Lane B.G., J.M. Dunwell, J.A. Ray, M.R. Schmitt & A.C. Cuming, 1993. Germin, a protein marker of early plant development, is an oxalate oxidase. J. Biol. Chem. 268: 12239–12242.

    PubMed  CAS  Google Scholar 

  • Marciano P., Di Lenna & P. Magro, 1983. Oxalic acid, cell wall degrading enzymes and pH in pathogenesis and their significance in the virulence of two Sclerotinia sclerotiorum isolates on sunflower. Physiol. Plant Pathol. 22: 339–345.

    CAS  Google Scholar 

  • Masirevic S. & T.J. Gulya, 1992. Sclerotinia and Phomopsis-two devastating sunflower pathogens. Field Crops Res. 30: 271–300.

    Article  Google Scholar 

  • Moloney M.M., J.M. Walker & K.K. Sharma, 1989. High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Reports 8: 238–242.

    Article  CAS  Google Scholar 

  • Noyes R.D. & J.G. Hancock, 1981. Role of oxalic acid in the Sclerotinia wilt of sunflower. Physiol. Plant Pathol. 18: 123–132.

    CAS  Google Scholar 

  • Rowe D.E., 1993. Oxalic acid effects in exudates of Sclerotinia trifoliorum and S. sclerotiorum and potential use in selection. Crop Sci. 33: 1146–1149.

    Article  CAS  Google Scholar 

  • Sackston W.E., 1992. On a treadmill: breeding sunflowers for resistance to disease. Ann. Rev. Phytopathol. 30: 529–551.

    Article  CAS  Google Scholar 

  • Schmitt M.R., 1991. Barley seedling oxalate oxidase: purification and properties. Plant Physiol. (suppl.) 96: 85.

    Google Scholar 

  • Sperry J.S. & M.T. Tyree, 1988. Mechanism of water stress-induced xylem embolism. Plant Physiol. 88: 581–587.

    Article  PubMed  CAS  Google Scholar 

  • Stefanov I., S. Fekete, L. Bogre, J. Pauk, A. Feher & D. Dudits, 1994. Differential activity of the mannopine synthase and the CaMV 35S promoters during development of transgenic rapeseed plants. Plant Sci. 95: 175–186.

    Article  CAS  Google Scholar 

  • Sugiura M., H. Yamamura, K. Hirano, M. Sasaki, M. Morikawa & M. Tsuboi, 1979. Purification and properties of oxalate oxidase from barley seedlings. Chem. Pharm. Bull. 27: 2003–2007.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thompson, C., Dunwell, J.M., Johnstone, C.E. et al. Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase. Euphytica 85, 169–172 (1995). https://doi.org/10.1007/BF00023945

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00023945

Key words

Navigation