Euphytica

, Volume 85, Issue 1, pp 169–172

Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase

Authors

  • C. Thompson
    • Plant BiotechnologyZeneca Seeds
  • J. M. Dunwell
    • Plant BiotechnologyZeneca Seeds
  • C. E. Johnstone
    • Plant BiotechnologyZeneca Seeds
  • V. Lay
    • Plant BiotechnologyZeneca Seeds
  • J. Ray
    • Plant BiotechnologyZeneca Seeds
  • M. Schmitt
    • American Cyanamid
  • H. Watson
    • Plant BiotechnologyZeneca Seeds
  • G. Nisbet
    • Plant BiotechnologyZeneca Seeds
Article

DOI: 10.1007/BF00023945

Cite this article as:
Thompson, C., Dunwell, J.M., Johnstone, C.E. et al. Euphytica (1995) 85: 169. doi:10.1007/BF00023945

Summary

Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.

Key words

Brassica napusdisease toleranceoxalic acidoxalate oxidaseSclerotinia sclerotiorumtransformation

Copyright information

© Kluwer Academic Publishers 1995