Plant Molecular Biology

, Volume 23, Issue 5, pp 995–1003

DNA sequence requirement of a TATA element-binding protein from Arabidopsis for transcription in vitro

  • Fujio Mukumoto
  • Susumu Hirose
  • Hidemasa Imaseki
  • Ken-ichi Yamazaki
Research Article

DOI: 10.1007/BF00021814

Cite this article as:
Mukumoto, F., Hirose, S., Imaseki, H. et al. Plant Mol Biol (1993) 23: 995. doi:10.1007/BF00021814

Abstract

We have analyzed the DNA sequence requirements for the functioning of TATA elements by examining the transcriptional activities associated with 24 promoters, including representatives of each of the 21 point mutations in the consensus sequence from plants, TATATATA, in a HeLa in vitro system and in a chimeric in vitro system in which human TATA-binding protein (hTBP) was replaced by purified TBP of Arabidopsis (aTBP-1). Although the relative transcriptional activities varied among these promoters, both systems gave virtually identical results. Among the mutant TATA elements, those with the sequences TAGAGATA and GAGAGAGA had undetectable activity. The rest had activities that ranged from 7% to 130% of the activity associated with the consensus element. These results suggest the functional conservation of TBP between plants and animals.

Key words

Arabidopsisin vitro transcriptionTATA elementTFIIDTBP/TATA element-binding protein

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Fujio Mukumoto
    • 1
  • Susumu Hirose
    • 2
  • Hidemasa Imaseki
    • 1
  • Ken-ichi Yamazaki
    • 1
  1. 1.School of Agricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.National Institute of GeneticsMishimajapan