Plant Molecular Biology

, Volume 23, Issue 4, pp 749–757

Constitutive or light-regulated expression of the rolC gene in transgenic potato plants has different effects on yield attributes and tuber carbohydrate composition


  • Matthias Fladung
    • Max-Planck-Institut für Züchtungsforschung, Abteilung Pflanzenzüchtung und Ertragsphysiologie
  • Agim Ballvora
    • Max-Planck-Institut für Züchtungsforschung, Abteilung Pflanzenzüchtung und Ertragsphysiologie
  • Thomas Schmülling
    • Universität Tübingen, Lehrstuhl für Allgemeine Genetik
Research Articles

DOI: 10.1007/BF00021530

Cite this article as:
Fladung, M., Ballvora, A. & Schmülling, T. Plant Mol Biol (1993) 23: 749. doi:10.1007/BF00021530


Tetraploid potato clones, transgenic for the rolC gene of Agrobacterium rhizogenes under control of the light-inducible ribulose bisphosphate carboxylase small subunit promoter (rbcS-rolC), were compared, with respect to yield attributes and tuber carbohydrates, with transformed and untransformed controls and with 35S-rolC transgenic potato plants. In rbcS-rolC plants, the expression of the rolC gene was located mainly in leaves, while in 35S-rolC plants transcripts were detected as well in shoots and roots. Phenotypically, rbcS-rolC transgenic plants were found to be slightly reduced in plant size with a few more tillers than control plants. Photosynthetic rate and chlorophyll content were significantly lower in all rolC transgenic plants irrespective of the type of construct used. Tuber yield was not significantly different between controls and rbcS-rolC transgenic plants, but was reduced in the 35S-rolC transformants. Sucrose level was unchanged in all rolC clones investigated, whereas fructose content was significantly enhanced in 35S-rolC transformants, but not in the plants expressing the rolC gene in aerial plant parts only. In both types of rolC transgenic plants, glucose content was lower than in controls, resulting in a significant reduction of reducing sugar in tubers. The results suggest a hormonal influence on the carbohydrate composition of potato tubers.

Key words

reducing sugarsrolCSolanum tuberosumtransgenic potatotuberization

Copyright information

© Kluwer Academic Publishers 1993