Plant Molecular Biology

, Volume 30, Issue 5, pp 973–982

Characterization of a cDNA encoding a proline-rich 14 kDa protein in developing cortical cells of the roots of bean (Phaseolus vulgaris) seedlings

Authors

  • Dong-Woog Choi
    • Department of Biology and Research Center for Cell DifferentiationSeoul National University
  • Jai Young Song
    • Department of Biology and Research Center for Cell DifferentiationSeoul National University
  • Young Myung Kwon
    • Department of Biology and Research Center for Cell DifferentiationSeoul National University
  • Sang-Gu Kim
    • Department of Biology and Research Center for Cell DifferentiationSeoul National University
Research Article

DOI: 10.1007/BF00020808

Cite this article as:
Choi, D., Song, J.Y., Kwon, Y.M. et al. Plant Mol Biol (1996) 30: 973. doi:10.1007/BF00020808

Abstract

A cDNA clone, corresponding to mRNAs preferentially expressed in the roots of bean (Phaseolus vulgaris L.) seedlings, was isolated. This clone contains a 381 bp open reading frame encoding a polypeptide of 13.5 kDa, designated PVR5 (Phaseolus vulgaris root 5). The amino acid sequence of this clone is rich in proline (13.5%) and leucine (12.7%) and shares significant amino acid sequence homology with root-specific and proline-rich proteins from monocots (maize and rice), and proline-rich proteins from dicots (carrot, oilseed rape, and Madagascar periwinkle). The precise biological roles of these polypeptides are unknown. PVR5 mRNA accumulation is developmentally regulated within the root, with high levels at the root apex and declining levels at distances further from the root tip. In situ hybridization shows that PVR5 mRNA specifically accumulates in the cortical ground meristem in which maximal cell division occurs. Southern blot analysis suggests that genomic DNA corresponding to PVR5 cDNA is encoded by a single gene or a small gene family.

Key words

cysteine-rich domain14 kDa proline-rich proteinroot-specific genePhaseolus vulgaris

Copyright information

© Kluwer Academic Publishers 1996