Article

Plant Molecular Biology

, Volume 16, Issue 2, pp 199-207

First online:

The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold

  • Christoph MaasAffiliated withInstitut für Genetik der Universität zu Köln
  • , Jürgen LaufsAffiliated withMax-Planck Institüt für Züchtungsforschung
  • , Sarah GrantAffiliated withMax-Planck Institüt für Züchtungsforschung
  • , Christian KorfhageAffiliated withInstitut für Genetik der Universität zu Köln
  • , Wolfgang WerrAffiliated withInstitut für Genetik der Universität zu Köln

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Abstract

Both exon 1 and intron 1 of the maize Shrunken-1 (Sh1) gene individually stimulate expression of reporter genes in transient gene expression experiments if present within the transcription unit. The Sh1 exon 1 mediates a 10-fold increase in activity when inserted at the 5′ end of the bacterial chloramphenicol transacetylase (CAT) marker gene in both monocot and dicot protoplasts. The Sh1 intron 1 enhances chimeric gene expression in rice and maize protoplasts approximately 100-fold but inhibits CAT expression in tobacco protoplasts. In combination, the stimulatory effects of Sh1 exon 1 and intron 1 are multiplicative in monocot protoplasts resulting in a final enhancement of up to 1000-fold compared to the unmodified CAT or luciferase marker genes.

Key words

enhanced gene expression exon intron maize rice