Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen maize cDNA clones can be used to detect elements of this variability. Similar levels of polymorphism are observed when genomic DNA is digested with any of a number of different restriction enzymes and probed with individual clones. When a clone is hybridized to genomic DNAs prepared from several different maize lines, a number of different alleles are often detected at a single locus. At the same time one clone can often detect more than one independently segregating locus by cross hybridization to related sequences at other loci. As expected these markers are inherited as simple codominant Mendelian alleles from one generation to the next and colinkage of these markers can be demonstrated in the progeny from a heterozygous parent. In similar studies with tomato, remarkably different results were found. Few RFPs were demonstrable among domestic Lycopersicon esculentum lines although a higher level of variability could be detected when comparing esculentum with its wild Lycopersicon relatives. These results are discussed in relation to the applied uses of RFPs in plant breeding as well as the inherent variability of different plant genomes.
- Aviv H, Leder P: Purification of biologically active globin messenger RNA by chromotography on oligothymidylic acid cellulose. Proc Nat Acad Sci USA 69: 1408–1412 1972.
- Barker D, Schafer M, White R: Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 36: 131–138, 1984.
- Birnboim HC: A rapid alkaline extraction method for the isolation of plasmid DNA. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, Vol. 100. Recombinant DNA Part B. Academic Press, New York, 1983, pp 243–255.
- Beckman JS, Soller M: Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67: 35–43, 1983.
- Burr B, Evola E, Burr FA, Bechman JS: The application of restriction fragment length polymorphism to plant breeding. In: Setlow JK, Hollaender A (eds) Genetic engineering principles and methods. Plenum Press, New York and London, 1983, Vol. 5, pp 45–59.
- Botstein D, White R, Skolnick M, Davis RW: Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314–331, 1980.
- Dagert M, Ehrlich SD: Prolonged incubation in calcium chloride improves the competence of Eschericia coli cells. Gene 6: 23–28, 1979.
- Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: version II. Pl Mol Biol Rep 1: 19–21, 1983.
- Gergen JP, Stern RH, Wensink PC: Filter replicas and permanent collections of recombinant DNA plasmids. Nucl Acids Res 7: 2115–2136, 1979.
- Goldberg RB, Galau GA, Britton RJ, Davidson EH: Nonrepetitive DNA sequence representation in sea urchin messenger RNA. Proc Nat Acad Sci USA 70: 3516–3520, 1973.
- Gruenbaum Y, Naveh-Many T, Cedar H, Razin A: Sequence specificity of methylation in higher plant DNA. Nature 292: 860–862, 1981.
- Gubler U, Hoffman BJ: A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269, 1983.
- Gusella JF, Wexler NS, Conneally PM, Naylor SL, Anderson MA, Tanzi RE, Watkins PC, Ottina K, Wallace MR, Sakaguichi AY, Young AB, Shoulson I, Bonilla E, Martin JB: A polymorhic DNA marker genetically linked to Huntington's disease. Nature 306: 234–238, 1983.
- Helentjaris T, Gesteland R: Evaluation of random cDNA clones as probes for human restriction fragment polymorphisms. J Mol Appl Genet 2: 237–247, 1983.
- Holmes D, Quigley M: A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114: 193–197, 1981.
- Little P, Annison G, Darling S, Williamson R, Cambar T, Model B: Model for antenatal diagnosis of B-thalassemia and other monogenic disorders by molecular analysis of linked DNA polymorphisms. Nature 285: 144–147, 1980.
- Maas R: An improved colony hybridization with significantly increased sensitivity for detection of single genes. Plasmid 10: 296–298, 1983.
- Martin C, Northcote DH: Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination. Planta 151: 197–198, 1981.
- Minty AJ, Caravatti M, Robert B, Cohen A, Daubas P, Weydert A, Gros F, Buckingham ME: Mouse actin messenger RNAs, construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse a-actin mRNA. J Biol Chem 256: 1008–1014, 1981.
- Phillips J, Panny S, Kazazian H, Bochun C, Scott C, Smith R, Prenatal diagnosis of sickle cell anemia by restriction endoclease analysis: Hind111 polymorphisms in v-globin genes extend applicability. Proc Nat Acad Sci USA 77: 2853–2856, 1980.
- Rhoades MM: Duplicate genes in maize. Amer Nat 85: 105–110, 1951.
- Rivin CJ, Zimmer EA, Cullis CA, Walbot V, Huynh T, Davis RW: Evaluation of genomic variability at the nucleic acid level. Pl Mol Biol Rep 1:9–16, 1983.
- Saghai-Maroof MA, Soliman KM, Jorgensen R, Allard RW: Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Submitted Proc Natl Acad Sci USA.
- Soller M, Beckman JS: Genetic polymorphism in varietal identification and genetic improvement. Theor Appl Genet 67:25–33, 1983.
- Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517, 1975.
- Tanksley S: Molecular markers in plant breeding. Pl Mol Biol Rep 1:3–8, 1983.
- Tanksley SD, Orton TJ (eds): Isozymes in plant genetics and breeding 1B. Elsevier, New York, 1983.
- Vieira J, Messing J: The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268, 1982.
- Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding
Plant Molecular Biology
Volume 5, Issue 2 , pp 109-118
- Cover Date
- Print ISSN
- Online ISSN
- Martinus Nijhoff, The Hague/Kluwer Academic Publishers
- Additional Links
- restriction fragment polymorphism
- plant genomic variability
- Industry Sectors