Plant Molecular Biology

, Volume 26, Issue 6, pp 1737–1746

Molecular characterization of rice genes specifically expressed in the anther tapetum

Authors

  • Tohru Tsuchiya
    • Institute for Cell Biology and Genetics, Faculty of AgricultureIwate University
    • Laboratory of Plant Breeding, Faculty of AgricultureTohoku University
  • Kinya Toriyama
    • Laboratory of Plant Breeding, Faculty of AgricultureTohoku University
  • Shin-ichiro Ejiri
    • Institute for Cell Biology and Genetics, Faculty of AgricultureIwate University
  • Kokichi Hinata
    • Laboratory of Plant Breeding, Faculty of AgricultureTohoku University
Research Article

DOI: 10.1007/BF00019488

Cite this article as:
Tsuchiya, T., Toriyama, K., Ejiri, S. et al. Plant Mol Biol (1994) 26: 1737. doi:10.1007/BF00019488

Abstract

In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

Key words

anther in situ hybridization promoter analysis rice tapetum transgenic tobacco

Copyright information

© Kluwer Academic Publishers 1994