Plant Molecular Biology

, Volume 23, Issue 3, pp 445–451

Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast

  • Mark H. Bell
  • Nigel G. Halford
  • John C. Ormrod
  • Dennis Francis
Research Article

DOI: 10.1007/BF00019293

Cite this article as:
Bell, M.H., Halford, N.G., Ormrod, J.C. et al. Plant Mol Biol (1993) 23: 445. doi:10.1007/BF00019293

Abstract

We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic virus 35S gene promoter. Eight of the nine plants showed altered leaf morphology, the lamina being lengthened and twisted and the interveinal regions being pocketed. One of these was sacrificed for analysis of the root meristem, where the cells were found to be significantly smaller than in the wild type. The other seven were grown on and showed precocious flowering, flowers being produced earlier and in significantly greater numbers than in the wild type. They also developed abnormal flowers on short stalks developing in a position normally occupied by the most proximal axillary bud of otherwise normal flower pedicels. The presence or absence of these phenotypes in the primary transformants and in the T2 generation was associated with the presence or absence of detectable levels of cdc25 transcripts.

Key words

cdc25cell cyclemitotic inducerplant developmenttransgenic tobacco

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Mark H. Bell
    • 1
  • Nigel G. Halford
    • 1
  • John C. Ormrod
    • 2
  • Dennis Francis
    • 3
  1. 1.Department of Agricultural SciencesUniversity of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research StationBristolEngland
  2. 2.ZENECA AgrochemicalsJealott's Hill Research StationBracknellEngland
  3. 3.School of Pure and Applied BiologyUniversity of WalesCardiffWales