Plant Molecular Biology

, Volume 32, Issue 3, pp 493–504

A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression

  • Oshra Blecher
  • Noa Erel
  • Isabelle Callebaut
  • Keren Aviezer
  • Adina Breiman
Research Article

DOI: 10.1007/BF00019101

Cite this article as:
Blecher, O., Erel, N., Callebaut, I. et al. Plant Mol Biol (1996) 32: 493. doi:10.1007/BF00019101

Abstract

A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.

Key words

cDNAexpressionFKBPPPIasewheat

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Oshra Blecher
    • 1
  • Noa Erel
    • 1
  • Isabelle Callebaut
    • 2
  • Keren Aviezer
    • 1
  • Adina Breiman
    • 1
  1. 1.Department of Botany, The George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael
  2. 2.Systèmes Moléculaires & Biologie Structurale, Laboratoire de Minéralogie-Cristallographie, Centre National de la Recherche ScientifiqueURA09 Universités Paris P6 and P7Parix Cedex 05France