Plant and Soil

, Volume 39, Issue 1, pp 205–207

Rapid determination of free proline for water-stress studies


  • L. S. Bates
    • Department of Grain Science and IndustryKansas State University
  • R. P. Waldren
    • Evapotranspiration LaboratoryKansas State University
  • I. D. Teare
    • Evapotranspiration LaboratoryKansas State University
Short Communication

DOI: 10.1007/BF00018060

Cite this article as:
Bates, L.S., Waldren, R.P. & Teare, I.D. Plant Soil (1973) 39: 205. doi:10.1007/BF00018060


Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.

Copyright information

© Martinus Nijhoff Publishers 1973