Article

Plant Molecular Biology

, Volume 12, Issue 1, pp 31-40

First online:

Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae

  • Margaret I. BoultonAffiliated withInstitute of Plant Science Research, John Innes Institute
  • , Wallace G. BuchholzAffiliated withAgrigenetics Advanced Science Company
  • , Melanie S. MarksAffiliated withInstitute of Plant Science Research, John Innes Institute
  • , Peter G. MarkhamAffiliated withInstitute of Plant Science Research, John Innes Institute
  • , Jeffrey W. DaviesAffiliated withInstitute of Plant Science Research, John Innes Institute

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Abstract

Parameters affecting the efficiency of agroinfection of maize streak virus (MSV) in maize have been determined. Monomeric units, cloned at a number of sites in the MSV genome were not infectious but multimeric units containing partial duplications were equally as infectious as complete tandem dimeric clones. Inoculation of tandem dimeric units conjugated into different strains of Agrobacterium showed that both A. tumefaciens and A. rhizogenes were able to transfer DNA to maize and this ability was Ti (or Ri) plasmid-specific. Nopaline strains of A. tumefaciens and both agropine and mannopine A. rhizogenes strains efficiently transferred MSV DNA to maize. A number of strains were capable of MSV DNA transfer to other members of the Gramineae, providing information which may be essential for Agrobacterium-mediated transformation of monocotyledonous plants.

Key words

agroinfection Agrobacterium strains maize streak virus Gramineae maize