Plant Molecular Biology

, Volume 15, Issue 5, pp 707–722

A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis

  • D. Lee
  • T. H. N. Ellis
  • L. Turner
  • R. P. Hellens
  • W. G. Cleary
Article

DOI: 10.1007/BF00016121

Cite this article as:
Lee, D., Ellis, T.H.N., Turner, L. et al. Plant Mol Biol (1990) 15: 707. doi:10.1007/BF00016121

Abstract

A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.

Key words

retrotransposonPisumsegregationPCRRFLPmapping

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • D. Lee
    • 1
  • T. H. N. Ellis
    • 1
  • L. Turner
    • 1
  • R. P. Hellens
    • 1
  • W. G. Cleary
    • 1
  1. 1.AFRC Institute of Plant ResearchJohn Innes InstituteNorwichUK