Plant Molecular Biology

, Volume 11, Issue 2, pp 147–160

Molecular analysis of the alcohol dehydrogenase gene family of barley

  • Martin Trick
  • Elizabeth S. Dennis
  • Kenneth J. R. Edwards
  • William J. Peacock
Article

DOI: 10.1007/BF00015667

Cite this article as:
Trick, M., Dennis, E.S., Edwards, K.J.R. et al. Plant Mol Biol (1988) 11: 147. doi:10.1007/BF00015667

Abstract

One partial and two complete genomic clones of the three loci specifying alcohol dehydrogenase (ADH) in barley were isolated by screening libraries with a maize Adh1 cDNA probe. Each gene is characterised by an intron arrangement similar to that of both maize Adh1 and Adh2, although two genes show an exon fusion. A comparison with the maize coding sequences unambiguously assorts the barley loci into an Adh1-like gene and two Adh2-like genes, indicating that an ancient gene duplication underlies the widespread occurrence of two Adh loci in higher plants. In the barley lineage there has been a further duplication-transposition of a progenitor “Adh2” locus to give rise to the extant three-gene system, with gene copies of different ancestry being closely linked. An Adh1 null-allele, Adh1-M9, has been cloned; the available sequence includes an intron with a missing acceptor splice signal. Two independent clones of one of the barley Adh2-like genes have an 18 bp in-frame deletion towards the 3′ end of the coding sequence. The barley Adh2-like genes are extensively diverged in their 5′ sequences apart from a conserved 15 bp motif in the mRNA leader region and sequences at the start of transcription. A sequence related to the hexanucleotide core of a regulatory element found in maize Adh1 and in other anaerobically induced plant genes is present in the 5′ region of barley Adh2.

Key words

alcohol dehydrogenasebarleyDNA sequencesgene duplication

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Martin Trick
    • 1
  • Elizabeth S. Dennis
    • 2
  • Kenneth J. R. Edwards
    • 1
  • William J. Peacock
    • 2
  1. 1.Department of GeneticsUniversity of CambridgeCambridgeUK
  2. 2.Division of Plant IndustryCSIROCanberraAustralia
  3. 3.AFRC Institute of Plant Science ResearchCambridge LaboratoryTrumpingtonUK