Plant Molecular Biology

, Volume 9, Issue 2, pp 121–126

urf13-T of T cytoplasm maize mitochondria encodes a 13 kD polypeptide

Authors

  • R. P. Wise
    • Department of Plant PathologyUniversity of Florida
  • A. E. Fliss
    • Department of Plant PathologyUniversity of Florida
  • D. R. Pring
    • Department of Plant PathologyUniversity of Florida
  • B. G. Gengenbach
    • Department of Agronomy and Plant GeneticsUniversity of Minnesota
Article

DOI: 10.1007/BF00015644

Cite this article as:
Wise, R.P., Fliss, A.E., Pring, D.R. et al. Plant Mol Biol (1987) 9: 121. doi:10.1007/BF00015644

Abstract

Polyspecific antibody to a 17 amino acid synthetic peptide from the maize T-cytoplasm urf13-T mitochondrial open reading frame immunoprecipitated a 13 kD polypeptide from 35S-methionine incorporations of T cytoplasm maize. Male-fertile, toxin-insensitive mutants in which the urf13-T sequence is deleted do not synthesize the 13 kD polypeptide. A mutant designated T-4, which carries a 5 bp insertion and a premature stop codon, synthesizes a truncated polypeptide, corresponding to an open reading frame of 8.3 kD. Thus the 13 kD polypeptide is trunctated or absent in mutants expressing male fertility and toxin insensitivity in T-cytoplasm maize.

Key words

cytoplasmic male sterilitydisease susceptibilityCochliobolus heterostrophusPhyllosticta maydis

Copyright information

© Martinus Nijhoff Publishers 1987