The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The newpPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322bom site for mobilization fromEscherichia coli toAgrobacterium, and the ColE1 and pVS1 plasmid origins for replication inE. coli and inAgrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use inAgrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ α-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.
Supplementary Material (0)
- An G: Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco tissue. Plant Physiol 81: 86–91 (1986).
- Becker D, Kemper E, Schell J, Masterson R: New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol 20: 1195–1197 (1992).
- Bevan M: BinaryAgrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
- Bevan MW, Flavell RB, Chilton MD: A chimeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184–187 (1983).
- Cado IC: Molecular mechanisms of crown gall tumorigenesis. Crit Rev Plant Sci 10: 1–32 (1991).
- Cangelosi GA, Best EA, Martinetti G, Nester EW: Genetic analysis ofAgrobacterium. Meth Enzymol 204: 384–397 (1991).
- Carrer H, Hockenberry TN, Svab Z, Maliga P: Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol Gen Genet 241: 49–56 (1993).
- Carrer H, Staub JM, Maliga P: Gentamycin resistance inNicotiana conferred by AAC(3)-I, a narrow substrate specificity acetyltransferase. Plant Mol Biol 17: 301–303 (1991).
- Chambers SP, Prior SE, Barstow DA, Minton NP: The pMTL nic- cloning vectors. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene 68: 139–149 (1988).
- Chinault AC, Blakesley VA, Roessler E, Willis DG, Smith CA, Cook RG, Fenwick RG: Characterization of transferable plasmids fromShigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides. Plasmid 15: 119–131 (1986).
- deFramond A, Barton K, Chilton MD: Mini-Ti: A vector strategy for plant genetic engineering. Bio/technology 1: 262–272 (1983).
- Deblaere R, Reynaerts A, Hofte H, Hernalsteens JP, Leemans J, VanMontagu M: Vectors for cloning in plant cells. Meth Enzymol 153: 277–292 (1987).
- Ditta G, Stanfield S, Corbin D, Helinski DR: Broad host range cloning system for gram negative bacteria: construction of a gene bank ofRhizobium meliloti. Proc Natl Acad Sci USA 77: 7347–7351 (1980).
- Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry YS, Galluppi GR, Goldberg SB, Hoffmann NL, Soo SC: Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80: 4803–4807 (1983).
- Herrera-Estrella L, DeBlock M, Messens E, Hernalstens JP, VanMontagu M, Schell J: Chimeric genes as dominant selectable markers in plant cells. EMBO J 2: 987–995 (1993).
- Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA: A binary plant vector strategy based on separation of vir- and T-region of theAgrobactrium tumefaciens Ti plasmid. Nature 303: 179–180 (1983).
- Hollingshead S, Vapnek D: Nucleotide sequence analysis of a gene encoding a streptomycin-spectinomycin adenyltransferase. Plasmid 13: 17–30 (1985).
- Holsters M, deWaele D, Depicker A, Messens E, VanMontagu M, Schell J: Transfection and transformation ofA. tumefaciens. Mol Gen Genet 163: 181–187 (1978).
- Hood EE, Helmer GL, Fraley RT, Chilton MD: The hypervirulance ofAgrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bact 168: 1291–1301 (1986).
- Hooykaas PJJ, Schilperoort RA:Agrobacterium and plant genetic engineering. Plant Mol Biol 19: 15–38 (1992).
- Horsch RB, Fry JE, Hoffman NL, Eicholtz D, Rogers SD, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1229–1231 (1985).
- Itoh Y, Haas D: Cloning vectors derived from thePseudomonas plasmid pVS1. Gene 36: 27–36 (1985).
- Itoh Y, Watson JM, Haas D, Leisinger T: Genetic and molecular characterization of thePseudomonas plasmid pVS1. Plasmid 11: 206–220 (1984).
- Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
- Jones JDG, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K: Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res 1: 285–297 (1992).
- Marton L, Browse J: Facile transformation ofArabidopsis. Plant Cell Rep 10: 235–239 (1991).
- Peralta EG, Hellmiss R, Ream W: Overdrive, a T-DNA transmission enhancer on theA. tumefaciens tumour-inducing plasmid. EMBO J 5: 1137–1142 (1986).
- Prentki P, Karch F, Iida S, Meyer J: The plasmid cloning vector pBR325 contains a 482 base-pair long inverted duplication. Gene 14: 289–299 (1981).
- Timmermans MCP, Maliga P, Vieira J, Messing J: The pFF plasmids: cassettes utilising CaMV sequences for expression of foreign genes in plants. J Biotechnol 14: 333–344 (1990).
- Watson N: A new revision of the sequence of plasmid pBR322. Gene 70: 399–403 (1988).
- Yadav NS, Vanderleyden J, Bennet DR, Barnes WM, Chilton MD: Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc Natl Acad Sci USA 79: 6322–6326 (1982).
- Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp 18 and pUC19 vectors. Gene 33: 103–119 (1985).
- Zambryski P, Depicker A, Kruger K, Goodman HM: Tumor induction byAgrobacterium tumefaciens: analysis of the boundaries of T-DNA. J Mol Appl Genet 1: 361–370 (1982).
- Zambryski PC: Chronicles from theAgrobacterium plant cell DNA transfer story. Annu Rev Plant Physiol Plant Mol Biol 43: 465–490 (1992).
About this Article
- The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
Plant Molecular Biology
Volume 25, Issue 6 , pp 989-994
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- binary vectors
- gentamycin resistance
- kanamycin resistance
- Industry Sectors