Plant Molecular Biology

, Volume 26, Issue 2, pp 657–665

Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene

  • Yong-Yoon Chung
  • Seong-Ryong Kim
  • David Finkel
  • Martin F. Yanofsky
  • Gynheung An
Research Article

DOI: 10.1007/BF00013751

Cite this article as:
Chung, YY., Kim, SR., Finkel, D. et al. Plant Mol Biol (1994) 26: 657. doi:10.1007/BF00013751

Abstract

Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2% identity to AGL2 and 44,4% identity to AP1. The MADS box region was the most homologous to other MADS-domain-containing proteins. Northern blot analysis indicated that the rice MADS gene was preferentially expressed in floral organs. In situ localization studies showed that the transcript was uniformly present in young flower primordia and later became localized in palea, lemma, and ovary. Ectopic expression of OsMADS1 with the CaMV 35S promoter in transgenic tobacco plants dramatically alters development, resulting in short, bushy, early-flowering plants with reduced apical dominance. These results suggest that the OsMADS1 gene is involved in flower induction and that it may be used for genetic manipulation of certain plant species.

Key words

apical dominanceearly floweringflower developmentMADS boxrice regulatory generice

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Yong-Yoon Chung
    • 1
  • Seong-Ryong Kim
    • 1
    • 2
  • David Finkel
    • 1
  • Martin F. Yanofsky
    • 3
  • Gynheung An
    • 1
  1. 1.Institute of Biological ChemistryWashington State UniversityPullmanUSA
  2. 2.Department of BiologySogang UniversitySeoulKorea
  3. 3.Department of Biology and Center for Molecular GeneticsUniversity of California, San DiegoLa JollaUSA