Biotests with phytoplankton assemblages. Growth limitation along temporal and spatial gradients
- Øivind Løvstad
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The development of eight different species (populations) along temporal and vertical gradients in several lakes was studied. Many populations had an exponential growth phase and a decline phase. The growth rate was often high during the exponential phase. Some species, e.g. Oscillatoria spp. and Synedra cf. acus, often also had a long stationary phase. The growth rate and the sinking rate of these populations were often very low.
Laboratory batch experiments with dilute phytoplankton populations were carried out to estimate the degree of growth limitation (L) for different populations sample from different lakes during the three growth phases. L was always low and often zero for populations initially in the exponential phase and always high for populations initially in the decline phase. The biotests also gave results that can help to explain the vertical distribution of Oscillatoria or Asterionella in three lakes investigated.
The results indicate that the growth rates and the development of the populations were dependent on the external chemical and physical conditions. The transition between the different growth phases seemed often to be dependent on the external nutrient conditions. P, N, Si and Fe were probably the most growth-limiting nutrients. The growth rate of some diatoms was probably limited directly or indirectly at high pH.
Laboratory biotests with natural populations may give valuable information on the growth-properties of different populations in the lakes. The biotests should, however, be carried out in combination with chemical and physical measurements and quantitative determinations of population densities.
- Ahlgren, G., 1980. Effects on algal growth rates by multiple nutrient limitation. Arch. Hydrobiol. 89: 43–53.
- Barlow, J. P., W. R. Schaffner, F. deNoyelles & B. J. Peterson, 1973. Continuous flow nutrient bioassays with natural phytoplankton populations. In Glass, G. E. (ed.). Bioassay Techniques and Environmental Chemistry.. Ann Arbor Science, Ann Arbor, Mich.: 229–319.
- Cescon, B. S. & P. G. Scarazzato, 1973. Determonation of low phosphate concentrations in seawater by an isobutyl acetate extraction procedure. Limnol. Oceanogr. 18: 499–500.
- deNoyelles, F. Jr. & W. J. O'Brien, 1978. Phytoplankton succession in nutrient enriched experimental ponds as related to changing carbon, nitrogen and phosphorus conditions. Arch. Hydrobiol. 84: 137–165.
- deNoyelles, F. Jr., R. Knoechel, D. Reinke, D. Treanor & C. Altenhofen, 1980. Continuous culturing of natural phytoplankton communities in The Experimental Lake Area: effects of enclosure, in situ incubation, light, phosphorus and cadmium. Can. J. Fish. aquat. Sci. 37: 424–433.
- Droop, M. R., 1968. Vitamin B12 and marine ecology IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri. J. mar. Biol. Ass. UK 48: 689–733.
- Droop, M. R., 1973. Some thoughts on nutrient limitation in algae. J. Phycol. 9: 264–272. CrossRef
- Droop, M. R., 1974. The nutrient status of algal cells in continuous culture. J. mar. Biol. Ass. UK 54: 825–855. CrossRef
- Gerhart, D. Z. & G. E. Likens, 1975. Enrichment experiments for determining nutrient limitation. Four methods compared. Limnol. Oceanogr. 20: 649–653.
- Goldman, C. R., 1978. The use of phytoplankton populations in bioassays. Mitt. int. Ver. Limnol. 21: 364–371.
- Golterman, H. L., 1969. Methods for Chemical Analysis of Fresh Waters. IBP Handbook 8. Blackwell Publications, Oxford, 166 pp.
- Guillard, R. R. L. & C. J. Lorenzen, 1972. Yellow-green algae with chlorophyllide c. J. Phycol. 8: 10–14. CrossRef
- Harris, G. P., 1980. Temporal and spatial scales in phytoplankton ecology. Mechanisms, methods, models and management. Can. J. Fish. aquat. Sci. 37: 877–900. CrossRef
- Healey, F. P., 1979. Short-term responses of nutrient-deficient algae to nutrient addition. J. Phycol. 15: 289–299. CrossRef
- Holm, N. P. & D. E. Armstrong, 1981. Role of nutrient limitation and competition in controlling the population of Asterionella formosa and Microcystis aeruginosa in semicontinuous culture. Limnol. Oceanogr. 26: 622–634.
- Jannasch, H. W., 1974. Steady state and the chemostat in ecology. Limnol. Oceanogr. 19: 716–720.
- Jones, K. J., P. Tett, A. S. Wallis & B. J. B. Wood, 1978. Investigation of a nutrient-growth model using a continuous culture of natural phytoplankton. J. mar. Biol. Ass. UK 58: 923–941. CrossRef
- Kilham, S. S., 1975. Kinetics of silicon-limited growth in fresh-water diatom Asterionella formosa. J. Phycol. 11: 396–399. CrossRef
- Klaveness, D., 1977. Morphology, distribution and significance of the manganese-accumulating microorganism Metallogenium in lakes. Hydrobiologia 56: 25–33. CrossRef
- Løvstad, Ø., 1983. Determination of growth-limiting nutrients for red species of Oscillatoria and two ‘oligotrophic’ diatoms. Hydrobiologia 107: 221–230. CrossRef
- Løvstad, Ø., 1984a. Competitive ability of laboratory batch phytoplankton populations at limiting nutrient levels. OIKOS 42: 176–184.
- Løvstad, Ø., 1984b. Determination of growth-limiting factors for Oscillatoria agardhii Gom. and diatoms in eutrophic lakes. OIKOS 42: 185–192.
- Løvstad, Ø. & T. Wold, 1984. Determination of external concentrations of available phosphorus for phytoplankton populations. Verh. int. Ver. Limnol. 22: 205–210.
- Monod, J., 1950. La technique de culture continue: theorie et applications. Ann. Inst. Pasteur. 79: 390–410.
- Moss, B., 1969. Limitation of algal growth in some central African waters. Limnol. Oceanogr. 14: 591–601.
- Peterson, B. J., J. P. Barlow & A. Savage, 1974. The physiological state with respect to phosphorus of Cayuga Lake phytoplankton. Limnol. Oceanogr. 19: 396–408.
- Rhee, G-Y., 1974. Phosphate uptake under nitrate limitation by Scenedesmus sp. and its ecological implications. J. Phycol. 10: 470–475. CrossRef
- Rhee, G-F. & I. J. Gotham, 1980. Optimum N:P ratios and coexistence of planktonic algae. J. Phycol. 16: 486–489. CrossRef
- Rhee, G-Y. & I. J. Gotham, 1981a. The effect of environmental factors on phytoplankton growth: Temperature and the interactions of temperature with nutrient limitation. Limnol. Oceanogr. 26: 635–648. CrossRef
- Rhee, G-Y. & I. J. Gotham, 1981b. The effect of environmental factors on phytoplankton growth: Light and the interactions of light and nitrate limitation. Limnol. Oceanogr. 26: 649–659.
- Ryther, J. H. & R. R. L. Guillard, 1959. Enrichment experiments as a means of studying nutrients limiting to phytoplankton production. Deep-Sea Res. 6: 65–69.
- Schelske, C. L., E. D. Rotman, E. F. Stoermer & M. A. Santiago, 1974. Responses of phosphorus limited Lake Michigan phytoplankton to factorial enrichments with nitrogen and phosphorus. Limnol. Oceanogr. 19: 409–419.
- Tilman, D., 1977. Resource competition between planktonic algae: an experimental and a theoretical approach. Ecology 58: 338–348. CrossRef
- Tilman, D. & S. S. Kilham, 1976. Phosphate and silicate growth and uptake kinetics of the diatoms Asterionella formosa and Cyclotella meneghiniana in batch and semicontinuous culture. J. Phycol. 12: 375–383. CrossRef
- Utermöhl, H., 1958. Zur Vervollkommung der quantitativen Phytoplanktonmethodik. Mitt. int. Ver. Limnol. 9: 1–38.
- Biotests with phytoplankton assemblages. Growth limitation along temporal and spatial gradients
Volume 134, Issue 2 , pp 141-149
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- Kluwer Academic Publishers
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- phytoplankton populations
- growth limitation
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- Øivind Løvstad (1)
- Author Affiliations
- 1. Department of Limnology, University of Oslo, Blindern, PO Box 1027, 3, N-Oslo, Norway