Cortisol, glucose and hematocrit changes during acute stress, cohort sampling, and the diel cycle in diploid and triploid brook trout (Salvelinus fontinalis Mitchill)
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In this study, groups of diploid (control) and triploid brook trout were subjected to a 5 min acute handling/confinement stress. Blood samples were collected from equal numbers of diploid and triploid fish at regular predetermined intervals, beginning prior to confinement and continuing for up to 3h after confinement. These blood samples were used for the measurement of hematocrit and plasma cortisol and glucose concentrations. The effects of cohort sampling and the diel cycle on these variables were also analysed. Except for minor differences, both groups responded similarly to the acute stress, with short-term elevations in plasma cortisol and hematocrit levels, and a more sustained increase in plasma glucose levels. No significant differences were found between diploid and triploid fish in their reactions to cohort sampling. However, the act of removing an individual from a tank caused significant changes in plasma cortisol levels in cohorts remaining in that tank (p < 0.001). Significant diel effects were observed for both ploidy groups in plasma cortisol (p < 0.05) but not in plasma glucose or hematocrit.
- Audet, C. and Claireaux, G. 1992. Diel and seasonal changes in resting levels of various blood parameters in brook trout (Salvelinus fontinalis). Can. J. Fish. Aquat. Sci. 49: 870–877.
- Barton, B.A. and Iwama, G.K. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann. Rev. Fish Dis. 1: 3–26. CrossRef
- Barton, B.A., Peter, R.E. and Paulencu, C.R. 1980. Plasma cortisol levels of fingerling rainbow trout (Salmo gairdneri) at rest, and subjected to handling, confinement, transport and stocking. Can. J. Fish. Aquat. Sci. 37: 805–811. CrossRef
- Barton, B.A., Schreck, C.B., Ewing, R.D., Hemmingsen, A.R. and Patino, R. 1985. Changes in plasma cortisol during stress and smoltification in coho salmon, Oncorhynchus kisutch. Gen. Comp. Endocrinol. 59: 468–471. CrossRef
- Barton, B.A., Schreck, C.B. and Sigismondi, L.A. 1986. Multiple acute disturbances evoke cumulative physiological responses in juvenile chinook salmon. Trans. Am. Fish. Soc. 115: 245–251. CrossRef
- Benfey, T.J. 1991. The physiology of triploid salmonids in relation to aquaculture. Can. Tech. Rep. Fish. Aquat. Sci. 1789: 73–80.
- Benfey, T.J., Dye, H.M., Solar, I.I. and Donaldson, E.M. 1989. The growth and reproductive endocrinology of adult triploid Pacific salmonids. Fish Physiol. Biochem. 6: 113–120. CrossRef
- Benfey, T.J., Sutterlin, A.M. and Thompson, R.J. 1984. Use of erythrocyte measurements to identify triploid salmonids. Can. J. Fish. Aquat. Sci. 41: 980–984. CrossRef
- Biron, M. 1993. Primary and secondary responses to acute stress in triploid rainbow trout and brook trout. M.Sc. Thesis, University of New Brunswick, Fredericton, Canada.
- Bry, C. 1982. Daily variations in plasma cortisol levels of individual female rainbow trout Salmo gairdneri: evidence for a post-feeding peak in well-adapted fish. Gen. Comp. Endocrinol. 48: 462–468. CrossRef
- Davis, K.B. and Parker, N.C. 1983. Plasma corticosteroid and chloride dynamics in rainbow trout, Atlantic salmon, and lake trout during and after stress. Aquaculture 32: 189–194. CrossRef
- Donaldson, E.M. 1981. The pituitary-interrenal axis as an indicator of stress in fish. In Stress and Fish. Edited by A.D. Pickering. pp. 11–47. Academic Press, London.
- Flos, R., Reig, L., Torres, P. and Tort, L. 1988. Primary and secondary stress responses to grading and hauling in rainbow trout, Salmo gairdneri. Aquaculture 71: 99–106. CrossRef
- Klontz, G.W. 1979. Hematological techniques for fish. In Fish Health Management: II. Concepts and Methods of Fish Disease Epidemiology. pp. 100–130. Edited by G.W. Klontz. University of Idaho, Moscow.
- Korcock, D.E., Houston, A.H. and Gray, J.D. 1988. Effects of sampling conditions on selected blood variables of rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 33: 319–330. CrossRef
- Laidley, C.W. and Leatherland, J.F. 1988. Cohort sampling, anaesthesia and stocking-density effects on plasma cortisol, thyroid hormone, metabolite and ion levels in rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 33: 73–88. CrossRef
- Lincoln, R.F. and Scott, A.P. 1983. Production of all-female triploid rainbow trout. Aquaculture 30: 375–380. CrossRef
- Mazeaud, M.M., Mazeaud, F. and Donaldson, E.M. 1977. Primary and secondary effects of stress in fish: some new data with a general review. Trans. Am. Fish. Soc. 106: 201–212. CrossRef
- McCormick, S.D. and Naiman, R.J. 1984. Osmoregulation in the brook trout, Salvelinus fontinalis. I. Diel, photoperiod and growth related physiological changes in freshwater. Comp. Biochem. Physiol. 79A: 7–16. CrossRef
- Pickering, A.D. and Pottinger, T.G. 1983. Seasonal and diel changes in plasma cortisol levels of the brown trout, Salmo trutta L. Gen. Comp. Endocrinol. 49: 232–239. CrossRef
- Pickering, A.D., Pottinger, T.G. and Christie, P. 1982. Recovery of the brown trout, Salmo trutta L., from acute handling stress: a time-course study. J. Fish Biol. 20: 229–244. CrossRef
- Small, S.A. and Benfey, T.J. 1987. Cell size in triploid salmon. J. Exp. Zool. 241: 339–342. CrossRef
- Spieler, R.E. 1979. Diel rhythms of circulating prolactin, cortisol, thyroxine, and triiodothyronine levels in fishes: a review. Rev. Can. Biol. 38: 301–315.
- Spieler, R.E. and Noeske, T.A. 1984. Effects of photoperiod and feeding schedule on diel variations of locomotor activity, cortisol, and thyroxine in goldfish. Trans. Am. Fish. Soc. 113: 528–539. CrossRef
- Strange, R.J. and Schreck, C.B. 1978. Anesthetic and handling stress on survival and cortisol concentration in yearling chinook salmon (Oncorhynchus tshawytscha). J. Fish. Res. Bd. Can. 35: 345–349.
- Sumpter, J.P. and Donaldson, E.M. 1986. The development and validation of a radioimmunoassay to measure plasma ACTH levels in salmonid fishes. Gen. Comp. Endocrinol. 62: 367–376. CrossRef
- Sumpter, J.P., Dye, H.M. and Benfey, T.J. 1986. The effects of stress on plasma ACTH, α-MSH, and cortisol levels in salmonid fishes. Gen. Comp. Endocrinol. 62: 377–385. CrossRef
- Swarup, H. 1959. Effect of triploidy on the body size, general organization and cellular structure in Gasterosteus aculeatus (L.). J. Genet. 56: 143–155.
- Waring, C.P., Stagg, R.M. and Poxton, M.G. 1992. The effects of handling on flounder (Platichthys flesus L.) and Atlantic salmon (Salmo salar L.). J. Fish Biol. 41: 131–144. CrossRef
- Wedemeyer, G. 1976. Physiological response of juvenile coho salmon (Oncorhynchus kisutch) and rainbow trout (Salmo gairdneri) to handling and crowding stress in intensive fish culture. J. Fish. Res. Bd. Can. 33: 2699–2702.
- Woodward, C.C. and Strange, R.J. 1987. Physiological stress responses in wild and hatchery-reared rainbow trout. Trans. Am. Fish. Soc. 116: 574–579. CrossRef
- Cortisol, glucose and hematocrit changes during acute stress, cohort sampling, and the diel cycle in diploid and triploid brook trout (Salvelinus fontinalis Mitchill)
Fish Physiology and Biochemistry
Volume 13, Issue 2 , pp 153-160
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- cohort sampling
- diel periodicity
- brook trout