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Association of myeloperoxidase with ovarian cancer

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Tumor Biology

Abstract

Myeloperoxidase (MPO) is an oxidant generating enzyme normally restricted to myeloid cells, however aberrant MPO expression has been found to occur in non-myeloid cells in some disease states. The functional –463GA promoter polymorphism alters MPO expression levels. The –463G is within an SP1 binding site and is associated with higher gene expression. The G allele is most frequent with ~62 % of European populations being GG homozygotes. The GA polymorphism has been associated with risk or survival in a variety of cancers including lung and breast cancer. In this study we determined the frequency of the –463G/A polymorphism in 230 ovarian cancer patients, 75 patients with borderline ovarian tumors, and 299 healthy controls. The GG genotype was found to be overrepresented in patients with early stage ovarian cancer (83.3 % GG, p = 0.008) as compared to healthy controls (62 % GG), suggesting that MPO oxidants may increase risk. Immunohistochemical analysis revealed MPO expression in a subset of columnar ovarian epithelial carcinoma cells in early stage carcinomas.

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Acknowledgments

This project was supported by grants from the National Institutes of Health (R01 HL088428) and California Breast Cancer Research Program (161B) to WR, and from the Anniversary Fund of the Austrian National Bank for the Promotion of Scientific Research and Teaching (ÖNB 8864). We thank Marta Oleksjak and Michael Fischer (Medical University of Vienna, Austria), and the Department of Transfusion Medicine, University of Ulm, Germany, for providing the control samples.

Conflicts of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria

    Dan Cacsire Castillo-Tong, Alexander Reinthaller, Eva Schuster, Andrea Wolf, Rafal Watrowski & Robert Zeillinger

  2. Ludwig-Boltzmann Institute of Gynaecology and Gynaecologic Oncology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria

    Dietmar Pils

  3. Section of Clinical Biometrics, Core Unit of Medical Statistics and Informatics, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria

    Georg Heinze

  4. Department of Gynaecology and Obstetrics, Charité/Campus Virchow-Klinikum, University Medicine of Berlin, Berlin, Germany

    Ioana Braicu & Jalid Sehouli

  5. Sanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Richard A. Maki & Wanda F. Reynolds

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  1. Dan Cacsire Castillo-Tong
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Correspondence to Wanda F. Reynolds.

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Castillo-Tong, D.C., Pils, D., Heinze, G. et al. Association of myeloperoxidase with ovarian cancer. Tumor Biol. 35, 141–148 (2014). https://doi.org/10.1007/s13277-013-1017-3

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