Abstract
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. However, the molecular development of UM is not fully understood and current therapeutic modalities result in poor outcomes. Increasingly, data have shown that human papillomaviruses (HPVs) contribute to the development of cervical cancer and other malignancies, and the key viral oncoprotein E6/E7 has become the target of gene therapy in HPV-related cancers. In this study, we identified HPV 18 infection in the UM cell line, VUP, for the first time and silenced HPV 18 E6/E7 expression using siRNA. Our results demonstrated that down regulation of HPV 18E6/E7 led to growth inhibition and cell cycle block in VUP cells by activation of the p53 and Rb pathways. We propose that HPV is possibly involved in the development of UM, and provide a novel target for the development of therapeutic strategies for UM.
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Acknowledgments
This work was supported by the National Key Program for Basic Research of China grant (2010CB529902), The National Natural Science Foundation of China grant (81172323, 31200632). The Science and Technology Commission of Shanghai (10JC1409100, 12ZR1417300), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01040411), and the Shanghai Rising-Star Program (11QA1404000).
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Biyun Cun and Xin Song contributed equally.
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Cun, B., Song, X., Jia, R. et al. Cell growth inhibition in HPV 18 positive uveal melanoma cells by E6/E7 siRNA. Tumor Biol. 34, 1801–1806 (2013). https://doi.org/10.1007/s13277-013-0719-x
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DOI: https://doi.org/10.1007/s13277-013-0719-x