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Isolation and characterisation of ten microsatellite markers for the tetraploid Stypandra glauca R.Br. (Hemerocallidaceae) identified using next generation sequencing

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Abstract

Ten polymorphic microsatellite loci were developed for the tetraploid plant species Stypandra glauca which is common on granite outcrops in Western Australia. Amplification and genotyping trials were conducted on 48 individuals from two sampling localities. All 10 loci revealed multi-banding patterns with up to 4 bands visible in individuals consistent with tetraploidy. The number of alleles per locus ranged from 6 to 32 (mean = 12.5). The proportion of observed heterozygotes at each locus ranged from 0.32 to 0.98 (mean = 0.73). These polymorphic microsatellite markers will facilitate further analysis of population genetic structure and connectivity in Stypandra glauca.

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Authors and Affiliations

  1. Science Division, Department of Environment and Conservation, Bentley Delivery Centre, Locked Bag 104, Kensington, WA, 6983, Australia

    S.-L. Tapper & M. Byrne

  2. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia

    M. G. Gardner

  3. Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA, 5001, Australia

    M. G. Gardner

Authors
  1. S.-L. Tapper
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  2. M. Byrne
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  3. M. G. Gardner
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Correspondence to S.-L. Tapper.

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Tapper, SL., Byrne, M. & Gardner, M.G. Isolation and characterisation of ten microsatellite markers for the tetraploid Stypandra glauca R.Br. (Hemerocallidaceae) identified using next generation sequencing. Conservation Genet Resour 5, 529–531 (2013). https://doi.org/10.1007/s12686-012-9844-x

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  • DOI: https://doi.org/10.1007/s12686-012-9844-x

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