Abstract
We isolated and developed 18 novel microsatellite markers for the giant Pacific octopus (Enteroctopus dofleini) and examined them for 31 individuals from Prince William Sound (PWS), Alaska. These loci displayed moderate levels of allelic diversity (averaging 11 alleles per locus) and heterozygosity (averaging 65%). Seven loci deviated from Hardy–Weinberg Equilibrium (HWE) due to heterozygote deficiency for the PWS population, although deviations were not observed for all these loci in other populations, suggesting the PWS population is not in mutation-drift equilibrium. These novel microsatellite loci yielded sufficient genetic diversity for potential use in population genetics, individual identification, and parentage studies.
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Acknowledgments
We thank Sarah Sonsthagen and C. Roman Dial for laboratory and analytical assistance, and Gordon Scott, Reid Brewer, Robert Berceli, and Elisa Russ for samples. Henry Tomingas provided sampling equipment and boat use. Funding was provided by the At-Sea Processors Association Pollock Conservation Cooperative through Alaska Pacific University, the Alaska Space Grant Program, USGS Alaska Science Center, and Alaska Pacific University. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.
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Toussaint, R.K., Sage, G.K., Talbot, S.L. et al. Microsatellite marker isolation and development for the giant Pacific Octopus (Enteroctopus dofleini). Conservation Genet Resour 4, 545–548 (2012). https://doi.org/10.1007/s12686-011-9588-z
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DOI: https://doi.org/10.1007/s12686-011-9588-z