Skip to main content
Log in

Microsatellite marker isolation and development for the giant Pacific Octopus (Enteroctopus dofleini)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

We isolated and developed 18 novel microsatellite markers for the giant Pacific octopus (Enteroctopus dofleini) and examined them for 31 individuals from Prince William Sound (PWS), Alaska. These loci displayed moderate levels of allelic diversity (averaging 11 alleles per locus) and heterozygosity (averaging 65%). Seven loci deviated from Hardy–Weinberg Equilibrium (HWE) due to heterozygote deficiency for the PWS population, although deviations were not observed for all these loci in other populations, suggesting the PWS population is not in mutation-drift equilibrium. These novel microsatellite loci yielded sufficient genetic diversity for potential use in population genetics, individual identification, and parentage studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Barry P (2010) Examination of gear type efficacy, tagging methodology, and population structure for establishing a directed Enteroctopus dofleini fishery. School of Fisheries and Ocean Sciences. Master of Science, University of Alaska Fairbanks, Fairbanks, AK

  • Berry SS (1912) A review of the cephalopods of western North America. Bul Bur Fish 30(761):296–336

    Google Scholar 

  • Botstein D, White RI, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:182–190

    Google Scholar 

  • Buford M, Wayne R (1993) Microsatellites and their application to population genetic studies. Curr Opin Genet Dev 3:939–943

    Article  Google Scholar 

  • Guo SW, Thompson E (1992) Performing the exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics 48:361–372

    Article  PubMed  CAS  Google Scholar 

  • Hochberg FG (1998) Class cephalopoda. In: Scott PV, Blake JA (eds) Taxonomic atlas of the benthic fauna of the Santa Barbara basin and the western Santa Barbara channel. Santa Barbara California Santa Barbara Museum of Natural History 8. The Mollusca Part I: The Aplacophora Polyplacophora Scaphopoda Bivalvia and Cephalopoda, pp 175–236

  • Nixon M (1998) In: Voss NA, Vecchione M, Toll RB, Sweeney MJ (eds) Overview of cephalopod characters systematics and biogeography of cephalopods, vol I. Smithsonian Institution Press, Washington, DC, pp 7–9

  • Oetting WS, Lee HK, Flanders DJ, Wiesner GL, Sellers TA, King RA (1995) Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers. Genomics 30:450–458

    Article  PubMed  CAS  Google Scholar 

  • Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. PhD thesis, University of Dublin

  • Pickford GE (1964) Octopus dofleini (Wulker) the giant octopus of the North Pacific. Bull Bingham Oceanogr Collect 19:1–70

    Google Scholar 

  • Promega Corporation (2011) PowerStat (Version 1.2). http://www.promega.com/Fgeneticidtools/powerstats/

  • Raymond M, Rousset F (1995) GENEPOP (Version 12): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Reuter RF, Connors ME, Ducisumo J, Gaichas S, Ormseth O, Tenbrink T (2010) Managing non-target data-poor species using catch limits: lessons from the Alaskan groundfish fishery. Fish Manag Ecol 17:1–13

    Article  Google Scholar 

  • Scheel D (2002) Characteristics of habitats used by Enteroctopus dofleini in Prince William Sound and Cook Inlet Alaska PSZN. Mar Ecol 41(3):185–206

    Article  Google Scholar 

  • Stewart NC Jr, Via LE (1993) A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques 147:48–749

    Google Scholar 

  • Sunnucks P (2000) Efficient genetic markers for population biology. Trends Ecol Evol 15:199–203

    Article  PubMed  Google Scholar 

  • Valieré N (2003) GIMLET: a computer program for analyzing genetic individual identification data. Mol Ecol Notes 2:377–379

    Google Scholar 

  • Waits LP, Luikart G, Taberle P (2001) Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol Ecol 10:249–256

    Article  PubMed  CAS  Google Scholar 

  • Weir JL (1996) Genetic data analysis. Sinauer Associates, Sunderland

    Google Scholar 

Download references

Acknowledgments

We thank Sarah Sonsthagen and C. Roman Dial for laboratory and analytical assistance, and Gordon Scott, Reid Brewer, Robert Berceli, and Elisa Russ for samples. Henry Tomingas provided sampling equipment and boat use. Funding was provided by the At-Sea Processors Association Pollock Conservation Cooperative through Alaska Pacific University, the Alaska Space Grant Program, USGS Alaska Science Center, and Alaska Pacific University. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. K. Toussaint.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toussaint, R.K., Sage, G.K., Talbot, S.L. et al. Microsatellite marker isolation and development for the giant Pacific Octopus (Enteroctopus dofleini). Conservation Genet Resour 4, 545–548 (2012). https://doi.org/10.1007/s12686-011-9588-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9588-z

Keywords

Navigation