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Isolation and characterization of 12 microsatellite loci in the Peruvian scallop Argopecten purpuratus and cross-species amplification in other scallop species (family Pectinidae)

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Abstract

Argopecten purpuratus is an economically important marine bivalve. Twelve microsatellite loci were isolated and characterized in 20 individuals of A. purpuratus collected in Isla Independencia (Pisco, Peru) and tested for cross-species amplification in other 4 scallops (family Pectinidae). Ten loci were polymorphic. The number of alleles per locus ranged from 2 to 13. The observed (H O) and expected (H E) heterozygosities ranged from 0.100 to 0.900 and from 0.185 to 0.903, respectively. Two loci showed significant deviation from Hardy–Weinberg equilibrium. A low rate of cross-species amplification was found.

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Acknowledgments

We thank Ruben Alfaro (National University of Trujillo, Peru), Elmer Ramos (National University of San Marcos, Peru), Kazuo Inaba (University of Tsukuba) and Linsheng Song (Institute of Oceanology, China) for providing scallop samples and Takuma Ozaki and Aya Yamada (Hokkaido University) for their helpful advice.

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Authors and Affiliations

  1. Laboratory of Aquaculture Genetics and Genomics, Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido, 041-8611, Japan

    Alan Marín, Takafumi Fujimoto & Katsutoshi Arai

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  1. Alan Marín
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  2. Takafumi Fujimoto
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  3. Katsutoshi Arai
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Correspondence to Alan Marín.

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Marín, A., Fujimoto, T. & Arai, K. Isolation and characterization of 12 microsatellite loci in the Peruvian scallop Argopecten purpuratus and cross-species amplification in other scallop species (family Pectinidae). Conservation Genet Resour 4, 179–182 (2012). https://doi.org/10.1007/s12686-011-9503-7

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  • DOI: https://doi.org/10.1007/s12686-011-9503-7

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