Abstract
We have developed thirteen polymorphic microsatellite loci for the newt Triturus pygmaeus using an enriched-library approach. We have detected 171 alleles in 60 individuals genotyped (mean number of alleles per locus was 13.15) in two different populations in the Doñana National Park, Southwestern Spain. Expected heterozygosities ranged from 0.16 to 0.956 in one population (Mata de los Domínguez) and from 0.1 to 0.959 in the other population (Navazo de la Higuera). The levels of polymorphism of the developed markers render them readily applicable for population genetic studies of diversity, structure, and migration.
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Acknowledgments
The Consejería de Economía, Innovación y Ciencia (Local government of Andalucía) has supported this study (P07-RNM-02928, PI Jordi Bascompte). We acknowledge to the staff of Doñana National Park and to the monitoring research team of the Reserva Biológica de Doñana, for technical support in the field. Thank you very much to Dave Daversa who helped during the last field trip. We are in debt with Isabel Máximo for her help with lab work and with Juanmi Arroyo and Cristina Rigueiro for technical advice. We also acknowledge the people who provided samples or kindly helped in their collection: Eva M. Aylagas, Maricel Aguilar, Jordi Bascompte and Kimberly Holbrook.
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Albert, E.M., Godoy, J.A. Characterization of 13 microsatellite loci for the Pygmy Marbled Newt Triturus pygmaeus (Salamandridae). Conservation Genet Resour 3, 745–747 (2011). https://doi.org/10.1007/s12686-011-9448-x
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DOI: https://doi.org/10.1007/s12686-011-9448-x