Abstract
Nine polymorphic microsatellites were isolated from a genomic DNA library of Oreochromis mossambicus enriched with di- and tetranucleotide repeats. The three di- and six tetranucleotide-type markers were characterized in 40 Mozambique tilapia individuals collected from a local farm. The number of alleles at the nine microsatellite loci ranged from 3 to 31, with an average of 13.4/locus. The average expected heterozygosity was 0.79 (range: 0.65–0.94), whereas the average observed heterozygosity was 0.67 (range: 0.33–1). Three loci significantly deviated from Hardy–Weinberg equilibrium, presumably due to limited population size. Cross-species amplification was successful for seven markers in Oreochromis niloticus, a phylogenetically closely related species and for six markers in Pseudotropheus lombardi, an ornamental cichlid for which no microsatellites have been described to date. These markers could provide important tools for the determination of genetic diversity and population structure of O. mossambicus.
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Acknowledgments
We acknowledge the technical help of David Bal in obtaining the tilapia samples, the advice provided by Grace Lin, Preethi Ravi, Felicia Feng, Dr. Chun Ming Wang and Dr. Gen Hua Yue and the help of Gandi Ng and Vidar Lund during marker characterization. Research in LO’s lab is financially supported by the Singapore Millenium Foundation and Temasek Holdings, Singapore.
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Saju, J.M., Lee, WJ. & Orban, L. Characterization of nine novel microsatellites isolated from Mozambique tilapia, Oreochromis mossambicus . Conservation Genet Resour 2 (Suppl 1), 385–387 (2010). https://doi.org/10.1007/s12686-010-9265-7
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DOI: https://doi.org/10.1007/s12686-010-9265-7