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Species identification method for marine products of Seriola and related species

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  • Food Science and Technology
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Abstract

The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S. rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphe japonica, and Rachycentron canadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a 373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species.

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Acknowledgments

This study was supported by a project grant (Development of Evaluation and Management Methods for Supply of Safe, Reliable and Functional Food and Farm Produce) from the National Food Research Institute and the Ministry of Agriculture, Forestry and Fisheries of Japan.

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Authors and Affiliations

  1. Food and Agricultural Materials Inspection Center, 2-1 Shintoshin, Chuo-ku, Saitama, 330-9731, Japan

    Jun Iguchi & Yasuharu Takashima

  2. Food and Agricultural Materials Inspection Center, Kobe Regional Center, 1-3-7 Minatoshima-minamimachi, Chuo-ku, Kobe, 651-0047, Japan

    Atsushi Namikoshi

  3. National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, 236-8648, Japan

    Michiaki Yamashita

Authors
  1. Jun Iguchi
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  2. Yasuharu Takashima
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  3. Atsushi Namikoshi
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  4. Michiaki Yamashita
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Correspondence to Jun Iguchi.

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Iguchi, J., Takashima, Y., Namikoshi, A. et al. Species identification method for marine products of Seriola and related species. Fish Sci 78, 197–206 (2012). https://doi.org/10.1007/s12562-011-0433-9

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  • DOI: https://doi.org/10.1007/s12562-011-0433-9

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