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Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

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Abstract

Plasma modification and plasma polymer deposition are valuable technologies for the preparation of surfaces for the covalent binding of biomolecules for applications such as biosensors, medical prostheses, and diagnostic devices as well as surfaces for enzyme-mediated reactions. Covalency is conveniently tested by the ability of the surface to retain the attached molecules after vigorous washing with sodium dodecyl sulphate (SDS). Covalency is indicated if the fraction of protein retained lies above the curve characteristic of physisorption. Confidence in covalency is strengthened when the washing protocol is aggressive enough to remove all adsorbed protein from a control significantly more hydrophobic than the test surface. The use of linker chemistry to space the molecules from the surface is in some cases beneficial. However, the use of linker chemistry is not necessary to retain molecular function for long periods when the polymer surface is modified by energetic bombardment. The energetic bombardment retains hydrophilicity of the surface by crosslinking the subsurface, and this appears to facilitate retention of protein function. Energetic bombardment also increases the functional life of molecules immobilized and then freeze dried on plasma-modified surfaces. Analysis of the surfaces shows that the covalent binding mechanism is related to the presence of free radicals on the surface and in the subsurface regions. The unpaired electrons associated with the radicals appear to be mobile within the modified region and can diffuse to the surface to take part in binding interactions. Proactive implantable devices can make use of these principles of covalent attachment by seeding the surface of an implant with a biomolecule that elicits the desired interaction with cells and prevents undesirable responses.

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Acknowledgements

The authors wish to acknowledge the Australian Research Council for financial support. We acknowledge also Professor Hans Griesser for helpful discussions on SDS washing as a test for covalent attachment of proteins to surfaces.

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  1. School of Physics, University of Sydney, Sydney, NSW, 2006, Australia

    Marcela M. Bilek & David R. McKenzie

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  1. Marcela M. Bilek
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  2. David R. McKenzie
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Correspondence to Marcela M. Bilek.

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Methods for Characterising Plasma Treated surfaces and their ability to strongly attach bioactive protein layers (DOC 64 kb)

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Bilek, M.M., McKenzie, D.R. Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants. Biophys Rev 2, 55–65 (2010). https://doi.org/10.1007/s12551-010-0028-1

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