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Oxidative stress in cystic fibrosis patients with Burkholderia cenocepacia airway colonization: relation of 8-isoprostane concentration in exhaled breath condensate to lung function decline

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Abstract

The association between oxidative stress and neutrophilic inflammation in cystic fibrosis (CF) lung disease is well recognized. 8-Isoprostane is a product of non-enzymatic oxidation of arachidonic acid. The aim of the present study was to examine the relationship between lung function decline and 8-isoprostane concentrations in exhaled breath condensate (EBC) in CF patients with Burkholderia cenocepacia airway colonization. Concentrations of 8-isoprostane in EBC were measured in 24 stable CF patients with B. cenocepacia airway colonization. The median (interquartile range) age of the cohort was 23.9 (22.0; 26.6) years. All patients underwent clinical examinations and pulmonary function tests at the time of EBC collection and in 1-, 3-, and 5-year intervals. 8-Isoprostane concentrations in EBC correlated to 1- and 3-year declines of forced expiratory volume in 1 s (FEV1) with r S values of −0.511 (p = 0.0011) and −0.495 (p = 0.016), respectively. In multiple regression analysis, 8-isoprostane concentrations in EBC were the only independent predictor for 1-year FEV1 decline (p = 0.01). When the median value of 8-isoprostane concentration in EBC (10.0 pg/mL) was used as a cutoff, subgroups of patients with lower and higher level of oxidative stress had significantly different median (interquartile range) FEV1 declines in 1-year interval, −2.4 % (−5.3; 0.8) and −7.3 % (−10.3; −5.8) predicted (p = 0.009). In conclusion, 8-isoprostane concentrations in EBC correlated to short-term lung function decline in CF patients with B. cenocepacia airway colonization. This correlation reflects the role of oxidative stress in CF lung pathogenesis and contributes to prediction of prognosis in these patients.

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Abbreviations

ATS:

American Thoracic Society

CF:

Cystic fibrosis

EBC:

Exhaled breath condensate

ERS:

European Respiratory Society

FEV1 :

Forced expiratory volume in 1 s

IQR:

Interquartile range

ROS:

Reactive oxygen species

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Acknowledgments

This study was supported by the project (Ministry of Health of the Czech Republic) for conceptual development of research organization 00064203 (University Hospital Motol, Prague, Czech Republic) and by grants from the Ministry of Health of the Czech Republic NS10543-3 and NT12405-5. The authors wish to thank Ms. Věra Lopotová for help with collection of EBC and preparation of samples for analyses, Ms. Venuše Flegrová for help with lung function tests, and Ms. Jana Prokopová for help with EBC analyses.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pneumology, Charles University, 2nd Faculty of Medicine, University Hospital Motol, V Úvalu 84, 150 06, Prague, Czech Republic

    Libor Fila & Alžběta Grandcourtová

  2. Department of Pharmacology, Charles University, Faculty of Medicine in Hradec Králové, Šimkova 870, 500 38, Hradec Kralové, Czech Republic

    Jaroslav Chládek

  3. Pulmonary Department, Hospital Mělník, Pražská 528, 276 01, Mělník, Czech Republic

    Jaromír Musil

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  1. Libor Fila
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  2. Alžběta Grandcourtová
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  3. Jaroslav Chládek
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  4. Jaromír Musil
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Correspondence to Libor Fila.

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Fila, L., Grandcourtová, A., Chládek, J. et al. Oxidative stress in cystic fibrosis patients with Burkholderia cenocepacia airway colonization: relation of 8-isoprostane concentration in exhaled breath condensate to lung function decline. Folia Microbiol 59, 217–222 (2014). https://doi.org/10.1007/s12223-013-0285-z

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  • DOI: https://doi.org/10.1007/s12223-013-0285-z

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