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An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda)

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Cell Stress and Chaperones Aims and scope

Abstract

The highly conserved part of the nucleotide-binding domain of the hsp70 gene family was amplified from the soil diplopod Tachypodoiulus niger (Julidae, Diplopoda). Genomic DNA yielded 701, 549 and 540 bp sequences, whereas cDNA from heat shocked animals produced only one distinct fragment of 543 bp. The sequences could be classified as a 70 kDa heat shock protein (hsp70), the corresponding 70 kDa heat shock cognate (hsc70) and a glucose-related hsp70 homologue (grp78). Comparisons of genomic and cDNA sequences of hsc70 identified two introns within the consensus sequence. Generally, stress-70 expression levels were low, which hampered successful RT-PCR and subsequent subcloning. Following experimental heat shock, however, the spliced hsc70 was amplified predominantly, instead of its inducible homologue hsp70. This finding suggests that microevolution in this soil-dwelling arthropod is directed towards low constitutive stress-70 levels and that the capacity for stress-70 induction presumably is limited. hsc70, albeit having introns, apparently is inducible and contributes to the stress-70 response.

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Acknowledgments

We gratefully acknowledge the support by D. Ammermann, N.K. Jacob, M. Knigge, T. Monsinjon and R. Paxton. The authors are also grateful to M. Bulant for assistance with the phylogenetic analysis. This study received funding from the German Research Council (Grant No. Ko 1978/2-1/2).

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Authors and Affiliations

  1. Laboratory of Ecotoxicology, EA 3222 PRES Normandie, Le Havre University, 25 Rue Philippe Lebon, F-76058, Le Havre Cedex, France

    Thomas Knigge

  2. Department of Research and Collections, Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318, Oslo, Norway

    Lutz Bachmann

  3. Animal Physiological Ecology, Institute of Evolution and Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, D-72072, Tübingen, Germany

    Heinz-R. Köhler

  4. Laboratoire d’Ecotoxicologie, Université du Havre, 25, rue Ph. Lebon – CS 80540, F-76058, Le Havre Cedex, France

    Thomas Knigge

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  1. Thomas Knigge
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Correspondence to Thomas Knigge.

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Knigge, T., Bachmann, L. & Köhler, HR. An intron-containing, heat-inducible stress-70 gene in the millipede Tachypodoiulus niger (Julidae, Diplopoda). Cell Stress and Chaperones 19, 741–747 (2014). https://doi.org/10.1007/s12192-014-0494-7

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  • DOI: https://doi.org/10.1007/s12192-014-0494-7

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