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Hepatitis B virus genotyping by enzyme-linked immunosorbent assay in Taiwan

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Abstract

Purpose

Restriction fragment length polymorphism (RFLP) and enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (mAbs) were used in this study to detect genotypes of HBV, and the efficiency and precision of ELISA using the mAbs for HBV genotype detection were also estimated.

Methods

The ELISA with mAbs method was used for the detection of HBV genotype in a Taiwanese population. The HBV genotypes of 100 chronic hepatitis B patients were determined by ELISA and were then compared with those obtained using RFLP.

Results

Genotype B was found to be the most prevalent in this study (63% by RFLP; 62% by ELISA) followed by genotype C (31% by RFLP; 35% by ELISA). There was no significant difference between the results obtained by RFLP and ELISA (P = 0.75). The ELISA overall genotypeable rate, the correct genotyping rate from genotypeable specimens, and the concordance of the HBV genotyping assay was 96.00, 94.79, and 91.00%; for the ELISA HBV genotyping assay for genotype B specimens was 96.77, 100.00, and 96.77%; and for genotype C specimens was 97.14, 91.18, and 88.57%, respectively. The mean HBV DNA level was higher in the specimens that could be genotyped by both RFLP and ELISA samples (6.24 ± 1.77 vs. 2.34 ± 0.90, log IU/ml), and a significant difference in terms of HBV DNA level of more than 2 × 103 IU/ml was identified between the genotyped RFLP samples (P < 0.001).

Conclusions

ELISA is a practical and a useful method for HBV genotyping in a clinical setting in Taiwan, in particular for patients with lower levels of HBV DNA.

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Acknowledgements

This study was supported by grants from the Taiwan Liver Research Foundation, Taiwan.

Conflict of interest

None.

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Authors and Affiliations

  1. Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

    Shu-Fen Liu, Chia-Yen Dai, Ming-Lung Yu & Wan-Long Chuang

  2. Department of Occupational Medicine, Kaohsiung Municipal HsiaoKang Hospital, Kaohsiung, Taiwan

    Meng-Hsuan Hsieh

  3. Department of Internal Medicine, Kaohsiung Municipal HsiaoKang Hospital, Kaohsiung, Taiwan

    Nai-Jen Hou & Jee-Fu Huang

  4. Faculty of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Ming-Yen Hsieh, Jee-Fu Huang, Chia-Yen Dai, Ming-Lung Yu & Wan-Long Chuang

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  1. Shu-Fen Liu
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  2. Meng-Hsuan Hsieh
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  3. Nai-Jen Hou
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  6. Chia-Yen Dai
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  8. Wan-Long Chuang
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Corresponding author

Correspondence to Chia-Yen Dai.

Additional information

S.-F. Liu and M.-H. Hsieh contributed equally to this study.

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Liu, SF., Hsieh, MH., Hou, NJ. et al. Hepatitis B virus genotyping by enzyme-linked immunosorbent assay in Taiwan. Hepatol Int 4, 601–607 (2010). https://doi.org/10.1007/s12072-010-9198-y

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  • DOI: https://doi.org/10.1007/s12072-010-9198-y

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