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MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1

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Abstract

MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours; however, little is known about its role in non-small cell lung cancer cells (NSCLCs). Here, we found that miR-200a was up-regulated in A549 and SK-MES-1 cells compared with normal lung cells HELF. By a series of gain-of-function and loss-of-function studies, over-expression of miR-200a was indicated to enhance cells migration, and its knock-down inhibited migration of cells in NSCLC cell lines. Furthermore, miR-200a was identified to induce TSPAN1 expression which was related to migration. TSPAN1 was proved to induce migration, and so up-regulation of TSPAN1 by miR-200a may explain why over-expressing miR-200a promotes NSCLC cells migration.

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Acknowledgements

We thank Professor Chen Li (Nantong University, Jiangsu, China) for providing TSPAN1 antibody and valuable suggestions on technology.

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Authors and Affiliations

  1. School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China

    Yaqing Chen & Tao Xi

  2. Biomics Biotechnologies Co. Ltd, Nantong, 226016, China

    Yaqing Chen, Wei Peng, Yixiang Lu, Jianxin Chen & York Yuanyuan Zhu

Authors
  1. Yaqing Chen
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  2. Wei Peng
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  3. Yixiang Lu
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  4. Jianxin Chen
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  5. York Yuanyuan Zhu
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  6. Tao Xi
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Corresponding authors

Correspondence to York Yuanyuan Zhu or Tao Xi.

Additional information

Corresponding editor: VEENA K PARNAIK

MS received 25 February 2013; accepted 17 June 2013

Corresponding editor: Veena K Parnaik

[Chen Y, Peng W, Lu Y, Chen J, Zhu YY And Xi T 2013 MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1. J. Biosci. 38 1–10] DOI 10.1007/s12038-013-9351-6

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Chen, Y., Peng, W., Lu, Y. et al. MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1. J Biosci 38, 523–532 (2013). https://doi.org/10.1007/s12038-013-9351-6

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  • DOI: https://doi.org/10.1007/s12038-013-9351-6

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