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Effects of pcDNA3-β-NGF Gene-modified BMSC on the Rat Model of Parkinson’s Disease

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Abstract

This study determined the effects of pcDNA3-β-nerve growth factor (NGF) gene-modified bone marrow stromal cells (BMSC) on the rat model of Parkinson’s disease (PD). The recombinant plasmid pcDNA3-β-NGF was transfected into BMSC, and NGF expression and its biological activity in vitro were detected. BMSC modified by the NGF gene were then grafted into the corpus striatum of PD rats, and the rotation behavior was evaluated at 1, 2, 4, and 6 weeks post-transplantation. A significant improvement in rotation behavior was observed in PD rats subjected to cell transplantation, especially in PD rats receiving NGF-modified BMSC. The genetically modified BMSC survived and expressed β-NGF but did not differentiate into tyrosine hydroxylase-positive cells in vivo. The present findings suggested that genetically modified BMSC could be effective for PD treatment, and the mechanisms might involve the neuroprotective effects of β-NGF.

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Acknowledgments

We thank Dr. S. K. Leong for his invaluable comments in the writing of this manuscript. This work was supported by the international collaboration grant of China Chunhui plan (Z2003-1-53013 and Z2005-1-53002), National Natural Science Foundation (no. 30660050) and the Chinese Medical Board of New York (CMB-00-72).

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Authors and Affiliations

  1. Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China

    Ting-Hua Wang

  2. Institute of Neuroscience, Kunming Medical College, no. 191, Renmin Western Road, Kunming, 650031, China

    Ting-Hua Wang, Zhong-Tang Feng, Peng Wei, Hui Li, Zhen-Jiang Shi & Li-Yan Li

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  1. Ting-Hua Wang
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  2. Zhong-Tang Feng
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  3. Peng Wei
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Correspondence to Ting-Hua Wang.

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The contribution of Zhong-Tang Feng is equal to the first author.

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Wang, TH., Feng, ZT., Wei, P. et al. Effects of pcDNA3-β-NGF Gene-modified BMSC on the Rat Model of Parkinson’s Disease. J Mol Neurosci 35, 161–169 (2008). https://doi.org/10.1007/s12031-007-9032-8

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  • DOI: https://doi.org/10.1007/s12031-007-9032-8

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