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Buffalo (Bubalus bubalis) Epiphyseal Proteins Counteract Arsenic-Induced Oxidative Stress in Brain, Heart, and Liver of Female Rats

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Abstract

Arsenic (As) toxicity through induction of oxidative stress is a well-known mechanism of organ toxicity. To address this problem, buffalo epiphyseal proteins (BEP, at 100 μg/kg BW, i.p. for 28 days) were administered intraperitoneally to female Wistar rats exposed to As (100 ppm sodium arsenite via drinking water for 28 days). Arsenic exposure resulted in marked elevation in lipid peroxidation in brain, cardiac, and hepatic tissues, whereas significant (p < 0.05) adverse change in catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and reduced glutathione level were observed in cardiac, hepatic, and brain tissues of As-administered animals. BEP significantly (p < 0.05) counteracted all the adverse changes in antioxidant defense system brought about by As administration. Based on these results, we consider BEP as a potent antioxidant to be used for protection from arsenic-induced oxidative stress related damage of vital organs.

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Acknowledgement

Research grant and facilities provided by Indian Veterinary Research Institute (IVRI) for conducting this study is duly acknowledged. We also acknowledge the tireless efforts of our lab and animal-shed assistants.

Conflict of Interest

All the authors declare that there are no conflicts of interest.

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Author notes

  1. Vijay K. Bharti

    Present address: Nutrition and Toxicology Laboratory, Defence Institute of High Altitude Research (DIHAR), Defence Research and Development Organization (DRDO), Ministry of Defence, C/o-56 APO, Leh, 194101, India

Authors and Affiliations

  1. Neurophysiology Laboratory Division of Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India

    Vijay K. Bharti & R. S. Srivastava

  2. Protein Chemistry Laboratory Division of Biochemistry and Food Science, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India

    B. Sharma

  3. Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India

    J. K. Malik

Authors
  1. Vijay K. Bharti
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  2. R. S. Srivastava
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  3. B. Sharma
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  4. J. K. Malik
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Correspondence to Vijay K. Bharti.

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Bharti, V.K., Srivastava, R.S., Sharma, B. et al. Buffalo (Bubalus bubalis) Epiphyseal Proteins Counteract Arsenic-Induced Oxidative Stress in Brain, Heart, and Liver of Female Rats. Biol Trace Elem Res 146, 224–229 (2012). https://doi.org/10.1007/s12011-011-9245-0

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  • DOI: https://doi.org/10.1007/s12011-011-9245-0

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