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Molecular Cloning, Tissue Expression, and Analysis with Genome DNA Methylation of Porcine LSD1 Gene

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Abstract

Lysine-specific demethylase 1 (LSD1) functioned as a demethyl methylase gene, underlying a wide range of biological processes, including cancer, cell apoptosis, differentiation, and development. To further understand the functions of the porcine LSD1 gene, we first obtained cDNA sequence of porcine LSD1 gene, using in silico cloning method. We further found that the porcine LSD1 gene has two transcripts, in which cDNA sequences are 2,716 and 2,656 bp, ORF are 2,622 and 2,562 bp, respectively. Then, RT-PCR analysis showed that the LSD1 gene is expressed in various tissues and relatively higher in the tissues of ovary, kidney, and spleen. Besides, the LSD1 gene was expressed higher in the growth nonage and peaked at 3 days in muscle tissue. Meanwhile, the expression of two transcript variants of LSD1 gene presented the same change trend. Besides, the level of DNA methylation was approximately fourfold higher in a 3-day muscle than in an old pig (180 days), significantly positive related to the gene expression of LSD1 (R = 0.9362, P < 0.05), and declined with growing age. Cloning, expression pattern, and analysis with genome DNA methylation of porcine LSD1 gene laid a foundation to clarify the molecular mechanisms of porcine growth and development and also for further work on animal breeding.

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Acknowledgments

This study was supported financially by National Key Technology R&D Program in the 12th Five Year Plan of China (2011BAD28B01-1), Special Fund for Agro-scientific Research in the Public Interest (201003036-02), Fundamental Research Funds for the Central Universities (2011QC001), students Research Fund (SRF) from Huazhong Agricultural University (1210504003), Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding (2010ZD111), and Science Foundation for Young Scholars of Hubei Academy of Agricultural Sciences (2011NKYJJ16).

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Authors and Affiliations

  1. Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Jin Chai, Lina Liu, Chen Chen, Yongdong Peng, Wei Jin, Rong Zheng & Siwen Jiang

  2. Hubei Key Laboratory of Amimal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science, 430070, Wuhan, China

    Qi Xiong

  3. Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Jian Peng

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  1. Jin Chai
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  2. Lina Liu
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  3. Qi Xiong
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  4. Chen Chen
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  5. Yongdong Peng
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  6. Wei Jin
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  7. Rong Zheng
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  8. Jian Peng
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  9. Siwen Jiang
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Correspondence to Jian Peng or Siwen Jiang.

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J. Chai and L.Liu have contributed equally to this work.

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Chai, J., Liu, L., Xiong, Q. et al. Molecular Cloning, Tissue Expression, and Analysis with Genome DNA Methylation of Porcine LSD1 Gene. Appl Biochem Biotechnol 169, 134–144 (2013). https://doi.org/10.1007/s12010-012-9966-3

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  • DOI: https://doi.org/10.1007/s12010-012-9966-3

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