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Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

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Abstract

In this paper, two new strians, Issatchenkia occidentalis (Lj-3, CCTCC M 2006097) and Issatchenkia orienalis (S-7, CCTCC M 2006098), isolated from different environments on solid media, were used in the detoxification process of the hemicellulosic hydrolysate of sugarcane bagasse. High-pressure liquid chromatography elution curve of UV-absorption compounds represented by acetic acid, furfural, and guaiacol (toxic compounds found in the hemicellulosic hydrolysate) showed that several chromatographic peaks were evidently diminished for the case of detoxified hydrolysate with isolate strains compared to the high peaks resulted for no detoxified hydrolysate. It was clear that these inhibitors were degraded by the two new isolates during their cultivation process. Fermentation results for the biodetoxified hydrolysate showed an increase in xylitol productivity (Q p) by 1.97 and 1.95 times (2.03 and 2.01 g l−1 h−1) and in xylitol yield (Y p) by 1.72 and 1.65 times (0.93 and 0.89 g xylitol per gram xylose) for hydrolysate treated with S-7 and Lj-3, respectively, in comparison with no detoxified hydrolysate (1.03 g l−1 h−1 and 0.54 g xylitol per gram xylose). This present work demonstrated the importance of Issatchenkia yeast in providing an effective biological detoxification approach to remove inhibitors and improve hydrolysate fermentability, leading to a high xylitol productivity and yield.

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Acknowledgements

This research was supported by the National Nature Science Foundation of China (No.30450007)

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Authors and Affiliations

  1. Phytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences, Yanshan, Guilin City, Guangxi Zhuang Autonomous Region, 541006, People’s Republic of China

    Zhang Hou-Rui, Qin Xiang-Xiang, Cai Ai-Hua, Zhou Yu-Heng & Jin Ke

  2. Biotechnology Department, Engineering School of Lorena-São Paulo University, Road Campinho, P.O. Box 116, Lorena, SP, Brazil

    Silvio S. Silva & Boutros F. Sarrouh

  3. Biotechnology Department, Gui Lin Medical College, Guilin City, Guangxi Zhuan Autonomous Region, 541004, People’s Republic of China

    Xiang Qiu

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  1. Zhang Hou-Rui
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  2. Qin Xiang-Xiang
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  3. Silvio S. Silva
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  4. Boutros F. Sarrouh
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  5. Cai Ai-Hua
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  6. Zhou Yu-Heng
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Correspondence to Zhang Hou-Rui.

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Hou-Rui, Z., Xiang-Xiang, Q., Silva, S.S. et al. Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate. Appl Biochem Biotechnol 152, 199–212 (2009). https://doi.org/10.1007/s12010-008-8249-5

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