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Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study

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Clinical Orthopaedics and Related Research®

Abstract

Osteonecrosis (ON) is commonly caused by high doses of glucocorticoids or ethanol intake. We previously reported suppression of BMP-2 by dexamethasone was more pronounced and enhancement by lovastatin was less pronounced in bone marrow stromal cells (BMSCs) from patients with ON than from patients without ON. We therefore presumed the BMSCs might be dysfunctional in patients with ON and performed this pilot study. We obtained BMSCs from 10 patients with ethanol-induced ON, 10 patients with glucocorticoid-induced ON, and 12 patients without ON as control subjects, checking third passage cells for osteogenic and adipogenic gene expression and differentiation ability. BMSCs from patients with glucocorticoid-induced ON possessed less osteogenic gene expression and less osteogenic differentiation, whereas BMSCs from patients with ethanol-induced ON possessed more adipogenic gene expression and more adipogenic differentiation. Dysfunction of BMSCs may be one of the causes of ON, with differing dysfunction in ethanol-induced ON and glucocorticoid-induced ON. Glucocorticoids may possess more of a suppressive effect on osteogenesis than ethanol, whereas ethanol may possess a more potent adipogenic effect than glucocorticoids on BMSCs.

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Acknowledgments

We thank Yi-Jen Chen for helping in the experiment process.

Author information

Authors and Affiliations

  1. Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Ching-Hua Yeh MS & Mei-Ling Ho PhD

  2. Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan

    Ching-Hua Yeh MS, Je-Ken Chang MD, Mei-Ling Ho PhD & Chung-Hwan Chen MD

  3. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Ching-Hua Yeh MS, Mei-Ling Ho PhD & Chung-Hwan Chen MD

  4. Center for Cervical Cancer Prevention, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

    Ching-Hua Yeh MS

  5. Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

    Ching-Hua Yeh MS

  6. Department of Orthopaedics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Je-Ken Chang MD & Chung-Hwan Chen MD

  7. Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Tzu-You 1st Road, Kaohsiung City, Taiwan

    Je-Ken Chang MD & Chung-Hwan Chen MD

  8. Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA

    Gwo-Jaw Wang MD

  9. Department of Orthopaedic Surgery, National Cheng-Kung University, No 100 Shi-Chuan 1st Road, Kaohsiung City, Taiwan

    Gwo-Jaw Wang MD

Authors
  1. Ching-Hua Yeh MS
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  2. Je-Ken Chang MD
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  3. Mei-Ling Ho PhD
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  4. Chung-Hwan Chen MD
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  5. Gwo-Jaw Wang MD
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Corresponding authors

Correspondence to Chung-Hwan Chen MD or Gwo-Jaw Wang MD.

Additional information

One or more of the authors (JKC, MLH, CHC, GJW) have received funding from the National Health Research Institute of Taiwan (NHRI-EX94-9316EP and NHRI-EX96-9615EP), Kaohsiung Medical University Hospital (93-KMUH-019), and the Technology Development Program for Academia in Taiwan (96-EC-17-A-17-S1-041).

Each author certifies that his or her institution has approved for the human protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

This work was performed at Kaohsiung Medical University Hospital.

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Yeh, CH., Chang, JK., Ho, ML. et al. Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study. Clin Orthop Relat Res 467, 2159–2167 (2009). https://doi.org/10.1007/s11999-009-0803-0

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  • DOI: https://doi.org/10.1007/s11999-009-0803-0

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