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Enhancing Osteochondral Allograft Viability: Effects of Storage Media Composition

  • Symposium: New Approaches to Allograft Transplantation
  • Published:
Clinical Orthopaedics and Related Research

Abstract

Osteochondral allograft transplantation is a well-accepted treatment for articular cartilage damage. However, chondrocyte viability declines during graft storage, which may compromise graft performance. We first tested the hypothesis that the composition of commonly used storage media affects the viability of articular chondrocytes over time; we then tested the hypothesis that the addition of insulin growth factor-1 or the apoptosis inhibitor ZVAD-fmk could enhance the storage properties of serum-free media. Bovine osteochondral grafts were stored at 4°C in lactated Ringer’s, Dulbecco’s modified eagle’s media (DMEM), DMEM supplemented with either insulin growth factor-1 or ZVAD-fmk, and a commercial storage media. Chondrocyte viability in lactated Ringer’s declined rapidly to 20.4% at 2 weeks. Viability in DMEM declined more slowly to 54.8% at 2 weeks and 31.2% at 3 weeks. Viability in commercial storage media was 83.6% at 3 weeks and 44.8% at 4 weeks. Viability was increased in DMEM + insulin growth factor-1 (56.4%) and DMEM + ZVAD (52.4%) at 3 weeks compared with DMEM alone. These results confirm the hypotheses that media composition greatly influences chondrocyte viability during cold storage and that insulin growth factor-1 and ZVAD improve the storage properties of DMEM.

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References

  1. Athanasiou KA, Rosenwasser MP, Buckwalter JA, Malinin TI, Mow VC. Interspecies comparisons of in situ intrinsic mechanical properties of distal femoral cartilage. J Orthop Res. 1991;9:330–340.

    Article  PubMed  CAS  Google Scholar 

  2. Ball ST, Amiel D, Wiliams SK, Tontz W, Chen AC, Sah RL, Bugbee WD. The effects of storage on fresh human osteochondral allografts. Clin Orthop Relat Res. 2004;418:246–252.

    Article  PubMed  Google Scholar 

  3. Costouros JG, Dang AC, Kim HT. Inhibition of chondrocyte apoptosis in vivo following osteochondral injury. Osteoarthritis Cartilage. 2003;11:756–759.

    Article  PubMed  CAS  Google Scholar 

  4. Csönge L, Bravo D, Newman-Gage H, Rigley T, Conrad EU, Bakay A, Strong DM, Pellet S. Banking of osteochondral allografts, part II. Preservation of chondrocyte viability during long-term storage. Cell Tissue Bank. 2002;3:161–168.

    Article  PubMed  Google Scholar 

  5. D’Lima DD, Hashimoto S, Chen PC, Lotz MK, Colwell CW Jr. Prevention of chondrocyte apoptosis. J Bone Joint Surg Am. 2001;83(Suppl 2):25–26.

    PubMed  Google Scholar 

  6. Kaplan LD. The analysis of articular cartilage after thermal exposure: is red really dead? Arthroscopy. 2003;19:310–313.

    PubMed  Google Scholar 

  7. Lightfoot A, Martin J, Amendola A. Fluorescent viability stains overestimate chondrocyte viability in osteoarticular allografts. Am J Sports Med. 2007;35:1817–1823.

    Article  PubMed  Google Scholar 

  8. Lo MY, Kim HT. Chondrocyte apoptosis induced by collagen degradation: inhibition by caspase inhibitors and IGF-1. J Orthop Res. 2004;22:140–144.

    Article  PubMed  CAS  Google Scholar 

  9. Loeser RF, Shanker G. Autocrine stimulation by insulin-like growth factor 1 and insulin-like growth factor 2 mediates chondrocyte survival in vitro. Arthritis Rheum. 2000;43:1552–1559.

    Article  PubMed  CAS  Google Scholar 

  10. Malinin T, Temple HT, Buck BE. Transplantation of osteochondral allografts after cold storage. J Bone Joint Surg Am. 2006;88:762–770.

    Article  PubMed  Google Scholar 

  11. Oates KM, Chen AC, Young EP, Kwan MK, Amiel D, Convery FR. Effect of tissue culture storage on the in vivo survival of canine osteochondral allografts. J Orthop Res. 1995;13:562–569.

    Article  PubMed  CAS  Google Scholar 

  12. Pearsall AW 4th, Tucker JA, Hester RB, Heitman RJ. Chondrocyte viability in refrigerated osteochondral allografts used for transplantation within the knee. Am J Sports Med. 2004;32:125–131.

    Article  PubMed  Google Scholar 

  13. Pennock AT, Wagner F, Robertson CM, Harwood FL, Bugbee WD, Amiel D. Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? J Knee Surg. 2006;19:265–272.

    PubMed  Google Scholar 

  14. Robertson CM, Allen RT, Pennock AT, Bugbee WD, Amiel D. Upregulation of apoptotic and matrix-related gene expression during fresh osteochondral allograft storage. Clin Orthop Relat Res. 2006;442:260–266.

    Article  PubMed  Google Scholar 

  15. Rohde RS, Studer RK, Chu CR. Mini-pig fresh osteochondral allografts deteriorate after 1 week of cold storage. Clin Orthop Relat Res. 2004;427:226–233.

    Article  PubMed  Google Scholar 

  16. Stockwell RA. The interrelationship of cell density and cartilage thickness in mammalian articular cartilage. J Anat. 1971;109:411–421.

    PubMed  CAS  Google Scholar 

  17. Wayne JS, Amiel D, Kwan MK, Woo SL, Fierer A, Meyers MH. Long-term storage effects on canine osteochondral allografts. Acta Orthop Scand. 1990;61:539–545.

    Article  PubMed  CAS  Google Scholar 

  18. Williams RJ 3rd, Dreese JC, Chen CT. Chondrocyte survival and material properties of hypothermically stored cartilage: an evaluation of tissue used for osteochondral allograft transplantation. Am J Sports Med. 2004;32:132–139.

    Article  PubMed  Google Scholar 

  19. Williams SK, Amiel D, Ball ST, Allen RT, Wong VW, Chen AC, Sah RL, Bugbee WE. Prolonged storage effects on the articular cartilage of fresh human osteochondral allografts. J Bone Joint Surg Am. 2003;85:2111–2120.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Stanford University School of Medicine, Stanford, CA, USA

    Margie S. Teng BS

  2. San Francisco VA Medical Center, San Francisco, CA, USA

    Audrey S. Yuen BS

  3. Orthopedic Surgery Section, San Francisco VA Medical Center, 4150 Clement Street (112), San Francisco, CA, 94121, USA

    Hubert T. Kim MD, PhD

Authors
  1. Margie S. Teng BS
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  2. Audrey S. Yuen BS
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  3. Hubert T. Kim MD, PhD
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Corresponding author

Correspondence to Hubert T. Kim MD, PhD.

Additional information

One or more of the authors (HTK) have received funding from the Musculoskeletal Transplant Foundation.

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Teng, M.S., Yuen, A.S. & Kim, H.T. Enhancing Osteochondral Allograft Viability: Effects of Storage Media Composition. Clin Orthop Relat Res 466, 1804–1809 (2008). https://doi.org/10.1007/s11999-008-0302-8

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  • DOI: https://doi.org/10.1007/s11999-008-0302-8

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