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Heat Shock Protein and Apoptosis in Supraspinatus Tendinopathy

  • Symposium: Molecular and Clinical Developments in Tendinopathy
  • Published:
Clinical Orthopaedics and Related Research

Abstract

Heat shock proteins (HSPs) are often upregulated following oxidative and other forms of stress. Based on reports of excessive apoptosis in torn supraspinatus tendon and mechanically loaded tendon cells, we hypothesized heat shock proteins may be present in rodent and human models of tendinopathy due to their central role in caspase dependent apoptotic cell signaling. We used a running rat supraspinatus tendinopathy overuse model with custom microarrays to investigate the process at a genetic level. Additionally torn supraspinatus tendon and matched intact subscapularis tendon samples were collected from patients undergoing arthroscopic shoulder surgery. Control samples of subscapularis tendon were collected from 10 patients undergoing arthroscopic stabilization surgery and evaluated using semiquantative RT-PCR and immunohistochemistry. We identified substantial upregulation of heat shock proteins and apoptotic genes in the rodent model. We further confirmed increased levels of heat shock protein and apoptotic regulatory genes in human supraspinatus and subscapularis tendon. This finding suggests heat shock proteins play a role in the cascade of stress-activated programmed cell death and degeneration in tendinopathy and may provide a novel target in preventing tendinopathies.

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Authors and Affiliations

  1. Orthopaedic Research Institute, Department of Orthopaedic Surgery, St George Hospital Campus, University of New South Wales, 4-10 South Street, Kogarah, NSW, 2217, Australia

    Neal L. Millar MBChB, MRCS (Glasg), Ai Q. Wei MBBS, Timothy J. Molloy PhD & George A. C. Murrell MD, DPhil

  2. Douglas Hanly Moir Pathology, Sydney, Australia

    Fiona Bonar MBChB, MRCPI, FRCPath(Lond)

Authors
  1. Neal L. Millar MBChB, MRCS (Glasg)
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  2. Ai Q. Wei MBBS
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  3. Timothy J. Molloy PhD
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  4. Fiona Bonar MBChB, MRCPI, FRCPath(Lond)
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  5. George A. C. Murrell MD, DPhil
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Correspondence to George A. C. Murrell MD, DPhil.

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Each author certifies that he or she has no commercial associations (eg, consultancies, stock ownership, equity interest, patent/licensing arrangements, etc) that might pose a conflict of interest in connection with the submitted article.

Each author certifies that his or her institution has approved the animal and human protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participation in the study was obtained.

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Millar, N.L., Wei, A.Q., Molloy, T.J. et al. Heat Shock Protein and Apoptosis in Supraspinatus Tendinopathy. Clin Orthop Relat Res 466, 1569–1576 (2008). https://doi.org/10.1007/s11999-008-0265-9

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  • DOI: https://doi.org/10.1007/s11999-008-0265-9

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